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Literature DB >> 11923344

Simultaneous differentiation and typing of Entamoeba histolytica and Entamoeba dispar.

Mehreen Zaki¹, Parool Meelu, Wei Sun, C Graham Clark.

Abstract

Sequences corresponding to some of the polymorphic loci previously reported from Entamoeba histolytica have been detected in Entamoeba dispar. Comparison of nucleotide sequences of two loci between E. dispar strain SAW760 and E. histolytica strain HM-1:IMSS revealed significant differences in both repeat and flanking regions. The tandem repeat units varied not only in sequence but also in number and arrangement between the two species at both the loci. Using the sequences obtained, primer pairs aimed at amplifying species-specific products were designed and tested on a variety of E. histolytica and E. dispar samples. Amplification results were in complete agreement with the original species classification in all cases, and the PCR products displayed discernible size and pattern variations among the isolates.

Entities: Species

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Year: 2002 PMID： 11923344 PMCID： PMC140395 DOI： 10.1128/JCM.40.4.1271-1276.2002

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

21 in total

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Journal: J Clin Microbiol Date: 1997-09 Impact factor: 5.948

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Journal: J Clin Microbiol Date: 2001-03 Impact factor: 5.948

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Journal: Am J Trop Med Hyg Date: 2001 May-Jun Impact factor: 2.345

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Journal: Trans R Soc Trop Med Hyg Date: 1995 Mar-Apr Impact factor: 2.184

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Journal: J Clin Microbiol Date: 1991-02 Impact factor: 5.948

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Journal: Trans R Soc Trop Med Hyg Date: 1989 Sep-Oct Impact factor: 2.184

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Authors: M Espinosa-Cantellano; A Gonzáles-Robles; B Chávez; G Castañ ón; C Argüello; A Lázaro-Haller; A Martínez-Palomo
Journal: J Eukaryot Microbiol Date: 1998 May-Jun Impact factor: 3.346

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Authors: L S Diamond; C G Clark
Journal: J Eukaryot Microbiol Date: 1993 May-Jun Impact factor: 3.346

29 in total

1. Prevalence and genotypic diversity of Entamoeba species in inhabitants in Kathmandu, Nepal.

Authors: Meng Feng; Kishor Pandey; Tetsuo Yanagi; Ting Wang; Chaturong Putaporntip; Somchai Jongwutiwes; Xunjia Cheng; Jeevan B Sherchand; Basu Dev Pandey; Hiroshi Tachibana
Journal: Parasitol Res Date: 2018-05-28 Impact factor: 2.289

2. Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geographic area.

Authors: Ali Haghighi; Seiki Kobayashi; Tsutomu Takeuchi; Gohta Masuda; Tomoyoshi Nozaki
Journal: J Clin Microbiol Date: 2002-11 Impact factor: 5.948

3. High prevalence of Entamoeba infections in captive long-tailed macaques in China.

Authors: Meng Feng; Bin Yang; Liu Yang; Yongfeng Fu; Yijun Zhuang; Longgan Liang; Qing Xu; Xunjia Cheng; Hiroshi Tachibana
Journal: Parasitol Res Date: 2011-04-12 Impact factor: 2.289

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Authors: Preetam H Shah; Ryan C MacFarlane; Dhruva Bhattacharya; John C Matese; Janos Demeter; Suzanne E Stroup; Upinder Singh
Journal: Eukaryot Cell Date: 2005-03

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Journal: Indian J Gastroenterol Date: 2012-08-19