Literature DB >> 11922595

Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis.

E Mortz1, T N Krogh, H Vorum, A Görg.   

Abstract

In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 11922595     DOI： 10.1002/1615-9861(200111)1:11<1359::AID-PROT1359>3.0.CO;2-Q

Source DB:  PubMed          Journal:  Proteomics        ISSN： 1615-9853            Impact factor:   3.984


  136 in total

1.  Intestinal Absorption of Fibrinolytic and Proteolytic Lumbrokinase Extracted from Earthworm, Eisenia andrei.

Authors:  Xiang Mei Yan; Chung-Hyo Kim; Chul Kyu Lee; Jang Sik Shin; Il Hwan Cho; Uy Dong Sohn
Journal:  Korean J Physiol Pharmacol       Date:  2010-04-30       Impact factor: 2.016

2.  Comparative studies of skeletal muscle proteome and transcriptome profilings between pig breeds.

Authors:  Nam-Kuk Kim; Hye-Ran Park; Hwi-Cheul Lee; Duhak Yoon; Eun-Suk Son; Yeun-Song Kim; Se-Ra Kim; Oun-Hyun Kim; Chang-Soo Lee
Journal:  Mamm Genome       Date:  2010-06-08       Impact factor: 2.957

3.  Discovery and verification of osteopontin and Beta-2-microglobulin as promising markers for staging human African trypanosomiasis.

Authors:  Natalia Tiberti; Alexandre Hainard; Veerle Lejon; Xavier Robin; Dieudonné Mumba Ngoyi; Natacha Turck; Enock Matovu; John Enyaru; Joseph Mathu Ndung'u; Alexander Scherl; Loïc Dayon; Jean-Charles Sanchez
Journal:  Mol Cell Proteomics       Date:  2010-08-19       Impact factor: 5.911

4.  Global organization and function of mammalian cytosolic proteasome pools: Implications for PA28 and 19S regulatory complexes.

Authors:  Toru Shibatani; Eric J Carlson; Fredrick Larabee; Ashley L McCormack; Klaus Früh; William R Skach
Journal:  Mol Biol Cell       Date:  2006-09-20       Impact factor: 4.138

5.  Comparative Proteomic Analysis of Plasma from Clinical Healthy Cows and Mastitic Cows.

Authors:  Yong-Xin Yang; Xing-Xu Zhao; Yong Zhang
Journal:        Date:  2009-10-28

6.  Schistosoma japonicum: proteomics analysis of differentially expressed proteins from ultraviolet-attenuated cercariae compared to normal cercariae.

Authors:  Lin-lin Yang; Zhi-yue Lv; Shao-min Hu; Si-jie He; Zheng-yu Li; Shuang-min Zhang; Huan-qin Zheng; Ming-tao Li; Xin-bing Yu; Ming-Chiu Fung; Zhong-dao Wu
Journal:  Parasitol Res       Date:  2009-03-17       Impact factor: 2.289

7.  Protein expression profile using two-dimensional gel analysis in squamous cervical cancer patients.

Authors:  Su-Mi Bae; Hyun-Jin Min; Guo Hua Ding; Sun-Young Kwak; Young-Lae Cho; Kye-Hyun Nam; Choong Hak Park; Yong-Wan Kim; Chong-Kook Kim; Byoung-Don Han; Young-Joo Lee; Do Kang Kim; Woong-Shick Ahn
Journal:  Cancer Res Treat       Date:  2006-04-30       Impact factor: 4.679

8.  Evidence that the xylanase activity from Sulfolobus solfataricus Oalpha is encoded by the endoglucanase precursor gene (sso1354) and characterization of the associated cellulase activity.

Authors:  Luisa Maurelli; Alfonso Giovane; Alessandra Esposito; Marco Moracci; Immacolata Fiume; Mosè Rossi; Alessandra Morana
Journal:  Extremophiles       Date:  2008-06-21       Impact factor: 2.395

9.  Comparison of mitochondrial and nucleolar RNase MRP reveals identical RNA components with distinct enzymatic activities and protein components.

Authors:  Qiaosheng Lu; Sara Wierzbicki; Andrey S Krasilnikov; Mark E Schmitt
Journal:  RNA       Date:  2010-01-19       Impact factor: 4.942

10.  Neutrophil MMP-9 proenzyme, unencumbered by TIMP-1, undergoes efficient activation in vivo and catalytically induces angiogenesis via a basic fibroblast growth factor (FGF-2)/FGFR-2 pathway.

Authors:  Veronica C Ardi; Philippe E Van den Steen; Ghislain Opdenakker; Bernhard Schweighofer; Elena I Deryugina; James P Quigley
Journal:  J Biol Chem       Date:  2009-07-16       Impact factor: 5.157
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.