F M Gakuya1, M N Kyule, P B Gathura, S Kariuki. 1. Department of Public Health, Pharmacology and Toxicology, University of Nairobi, P.O. Box 29053, Nairobi.
Abstract
OBJECTIVES: To determine if antimicrobial resistance occurs in E. coli isolated from rats and if this resistance is transferable via plasmids. METHODS: Sixty Escherichia coli isolates obtained from 215 rats trapped in and around Nairobi, Kenya, were analysed for antimicrobial susceptibility to eleven commonly used antimicrobials. Plasmid DNA analysis and in-vitro conjugation tests were conducted on twenty two resistant isolates. RESULTS: A total of 12 (20%) E. coli were resistant to at least one of the commonly used drugs including ampicillin, sulphamethoxazole and streptomycin. Eight (13.3%) isolates were multidrug resistant. The drug resistant E. coli fell into five plasmid profile groups with plasmids ranging between two and 98 megadaltons (Mda). Resistance to ampicillin was transferable. CONCLUSION: The results of this study show that rats from the study area may form an important reservoir for drug-resistant E. coli that could pose a public health hazard.
OBJECTIVES: To determine if antimicrobial resistance occurs in E. coli isolated from rats and if this resistance is transferable via plasmids. METHODS: Sixty Escherichia coli isolates obtained from 215 rats trapped in and around Nairobi, Kenya, were analysed for antimicrobial susceptibility to eleven commonly used antimicrobials. Plasmid DNA analysis and in-vitro conjugation tests were conducted on twenty two resistant isolates. RESULTS: A total of 12 (20%) E. coli were resistant to at least one of the commonly used drugs including ampicillin, sulphamethoxazole and streptomycin. Eight (13.3%) isolates were multidrug resistant. The drug resistant E. coli fell into five plasmid profile groups with plasmids ranging between two and 98 megadaltons (Mda). Resistance to ampicillin was transferable. CONCLUSION: The results of this study show that rats from the study area may form an important reservoir for drug-resistant E. coli that could pose a public health hazard.
Authors: Sylvia Omulo; Samuel M Thumbi; M Kariuki Njenga; Douglas R Call Journal: Antimicrob Resist Infect Control Date: 2015-01-28 Impact factor: 4.887
Authors: Richard Onanga; Pierre Philippe Mbehang Nguema; Guy Roger Ndong Atome; Arsène Mabika Mabika; Berthelemy Ngoubangoye; Wed Leslie Komba Tonda; Jean Constant Obague Mbeang; Jacques Lebibi Journal: Vet Med Int Date: 2020-07-18