Literature DB >> 11914362

Effect of aniA (carbon flux regulator) and PhaC (poly-beta-hydroxybutyrate synthase) mutations on pyruvate metabolism in Rhizobium etli.

Michael F Dunn1, Gisela Araíza, Sergio Encarnación, María del Carmen Vargas, Jaime Mora.   

Abstract

The Rhizobium etli poly-beta-hydroxybutyrate synthase (PhaC) mutant SAM100 grows poorly with pyruvate as the carbon source. The inactivation of aniA, encoding a global carbon flux regulator, in SAM100 restores growth of the resulting double mutant (VEM58) on pyruvate. Pyruvate carboxylase (PYC) activity, pyc gene transcription, and holoenzyme content, which were low in SAM100, were restored in strain VEM58. The genetically engineered overexpression of PYC in SAM100 also allowed its growth on pyruvate. The possible relation between AniA, pyc transcription, and reduced-nucleotide levels is discussed.

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Year:  2002        PMID： 11914362      PMCID： PMC134968          DOI： 10.1128/JB.184.8.2296-2299.2002

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  22 in total

1.  Site-directed mutagenesis and DNA sequence of pckA of Rhizobium NGR234, encoding phosphoenolpyruvate carboxykinase: gluconeogenesis and host-dependent symbiotic phenotype.

Authors:  M Osterås; T M Finan; J Stanley
Journal:  Mol Gen Genet       Date:  1991-11

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  AniA regulates reserve polymer accumulation and global protein expression in Rhizobium etli.

Authors:  Sergio Encarnación; María del Carmen Vargas; Michael F Dunn; Araceli Dávalos; Guillermo Mendoza; Yolanda Mora; Jaime Mora
Journal:  J Bacteriol       Date:  2002-04       Impact factor: 3.490

4.  The physiological effects and metabolic alterations caused by the expression of Rhizobium etli pyruvate carboxylase in Escherichia coli.

Authors:  R R Gokarn; J D Evans; J R Walker; S A Martin; M A Eiteman; E Altman
Journal:  Appl Microbiol Biotechnol       Date:  2001-07       Impact factor: 4.813

5.  Azorhizobium caulinodans pyruvate dehydrogenase activity is dispensable for aerobic but required for microaerobic growth.

Authors:  David C Pauling; Jerome P Lapointe; Carolyn M Paris; Robert A Ludwig
Journal:  Microbiology       Date:  2001-08       Impact factor: 2.777

6.  A critical role for aniA in energy-carbon flux and symbiotic nitrogen fixation in Sinorhizobium meliloti.

Authors:  S Povolo; S Casella
Journal:  Arch Microbiol       Date:  2000 Jul-Aug       Impact factor: 2.552

7.  Cloning and characterization of the pyruvate carboxylase from Sinorhizobium meliloti Rm1021.

Authors:  M F Dunn; G Araíza; T M Finan
Journal:  Arch Microbiol       Date:  2001-11       Impact factor: 2.552

8.  Ammonia assimilation and synthesis of alanine, aspartate, and glutamate in Methanosarcina barkeri and Methanobacterium thermoautotrophicum.

Authors:  W R Kenealy; T E Thompson; K R Schubert; J G Zeikus
Journal:  J Bacteriol       Date:  1982-06       Impact factor: 3.490

9.  Expression of the biotin biosynthetic operon of Escherichia coli is regulated by the rate of protein biotination.

Authors:  J E Cronan
Journal:  J Biol Chem       Date:  1988-07-25       Impact factor: 5.157

10.  Poly-beta-hydroxybutyrate turnover in Azorhizobium caulinodans is required for growth and affects nifA expression.

Authors:  K Mandon; N Michel-Reydellet; S Encarnación; P A Kaminski; A Leija; M A Cevallos; C Elmerich; J Mora
Journal:  J Bacteriol       Date:  1998-10       Impact factor: 3.490
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  4 in total

1.  argC Orthologs from Rhizobiales show diverse profiles of transcriptional efficiency and functionality in Sinorhizobium meliloti.

Authors:  Rafael Díaz; Carmen Vargas-Lagunas; Miguel Angel Villalobos; Humberto Peralta; Yolanda Mora; Sergio Encarnación; Lourdes Girard; Jaime Mora
Journal:  J Bacteriol       Date:  2010-11-12       Impact factor: 3.490

2.  AniA regulates reserve polymer accumulation and global protein expression in Rhizobium etli.

Authors:  Sergio Encarnación; María del Carmen Vargas; Michael F Dunn; Araceli Dávalos; Guillermo Mendoza; Yolanda Mora; Jaime Mora
Journal:  J Bacteriol       Date:  2002-04       Impact factor: 3.490

3.  Influence of the poly-3-hydroxybutyrate (PHB) granule-associated proteins (PhaP1 and PhaP2) on PHB accumulation and symbiotic nitrogen fixation in Sinorhizobium meliloti Rm1021.

Authors:  Chunxia Wang; Xiaoyan Sheng; Raymie C Equi; Maria A Trainer; Trevor C Charles; Bruno W S Sobral
Journal:  J Bacteriol       Date:  2007-10-05       Impact factor: 3.490

4.  Poly-beta-hydroxybutyrate biosynthesis in the facultative methylotroph methylobacterium extorquens AM1: identification and mutation of gap11, gap20, and phaR.

Authors:  Natalia Korotkova; Ludmila Chistoserdova; Mary E Lidstrom
Journal:  J Bacteriol       Date:  2002-11       Impact factor: 3.490
  4 in total

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