| Literature DB >> 1191264 |
J Sinclair, R Wells, B Deumling, J Ingle.
Abstract
Purified satellite DNA from melon (Cucumis melo) was shown to contain at least two components from thermal-denaturation and renaturation studies. Two components were separated after partial renaturation, a fast-renaturing fraction similar in complexity to mouse satellite DNA, and one with 6000 times greater complexity. Both components renatured very accurately, indicating a minimum of sequence divergence. Centrifugation of the purified satellite DNA in Ag+/Cs2SO4 gradients resolved two major and several minor fractions. The two major fractions were only slightly enriched for fast- or slow-renaturing sequences.Entities:
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Year: 1975 PMID: 1191264 PMCID: PMC1165589 DOI: 10.1042/bj1490031
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857