P I Chou1, D W Lu, J T Chen. 1. Department of Ophthalmology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China. sib00170@tpts1.seed.net.tw
Abstract
PURPOSE: To demonstrate the phenomenon of adrenergic denervation supersensitivity in rabbit choroidal blood vessels after superior cervical ganglionectomy. METHODS: Twenty four albino rabbits of both sexes weighing 2-3 kg were randomly separated into two groups. Twelve rabbits received bilateral superior cervical sympathectomy 2 weeks prior to the study (group s). The other 12 rabbits served as controls (group n). Four different concentrations of 0.1 ml phenylephrine, 0.05%, 0.025%, 0.013%, and 0.007% were slowly injected into the vitreous body near the retinal surface in group (n) and (s) rabbits (n = 6 in each group). The choroidal blood flow (PF), blood volume (CMBC), and velocity (V) were measured simultaneously by laser Doppler flowmetry (Perimed, PF 4001). RESULTS: The PF showed similar decreases in group (n) and (s) rabbits after injection of 0.05%, 0.025%, and 0.013% phenylephrine. With 0.007% phenylephrine, the PF remained unchanged in group (n) rabbits, but decreased significantly in group (s) rabbits (p = 0.0007). Velocity decreased similarly in both group (n) and (s) rabbits except for the 0.007% phenylephrine, concentration in which velocity decreased significantly in group s rabbits (p = 0.0001). There was no statistical difference in CMBC between group n and s rabbits at any of the test concentrations. CONCLUSIONS: The difference in PF decrease between group (n) and (s) rabbits with 0.007% phenylephrine demonstrated the existence of choroidal blood vessel denervation supersensitivity. The decrease in PF was achieved mainly through a decrease in blood cell velocity.
PURPOSE: To demonstrate the phenomenon of adrenergic denervation supersensitivity in rabbit choroidal blood vessels after superior cervical ganglionectomy. METHODS: Twenty four albino rabbits of both sexes weighing 2-3 kg were randomly separated into two groups. Twelve rabbits received bilateral superior cervical sympathectomy 2 weeks prior to the study (group s). The other 12 rabbits served as controls (group n). Four different concentrations of 0.1 ml phenylephrine, 0.05%, 0.025%, 0.013%, and 0.007% were slowly injected into the vitreous body near the retinal surface in group (n) and (s) rabbits (n = 6 in each group). The choroidal blood flow (PF), blood volume (CMBC), and velocity (V) were measured simultaneously by laser Doppler flowmetry (Perimed, PF 4001). RESULTS: The PF showed similar decreases in group (n) and (s) rabbits after injection of 0.05%, 0.025%, and 0.013% phenylephrine. With 0.007% phenylephrine, the PF remained unchanged in group (n) rabbits, but decreased significantly in group (s) rabbits (p = 0.0007). Velocity decreased similarly in both group (n) and (s) rabbits except for the 0.007% phenylephrine, concentration in which velocity decreased significantly in group s rabbits (p = 0.0001). There was no statistical difference in CMBC between group n and s rabbits at any of the test concentrations. CONCLUSIONS: The difference in PF decrease between group (n) and (s) rabbits with 0.007% phenylephrine demonstrated the existence of choroidal blood vessel denervation supersensitivity. The decrease in PF was achieved mainly through a decrease in blood cell velocity.
Authors: David J Miller; S Kevin Li; Anthony L Tuitupou; Rajan P Kochambilli; Kongnara Papangkorn; Donald C Mix; William I Higuchi; John W Higuchi Journal: J Ocul Pharmacol Ther Date: 2008-08 Impact factor: 2.671
Authors: Mustafa Gök; V Levent Karabas; Ender Emre; Arzu Toruk Aksar; Mehmet S Aslan; Dilek Ural Journal: Indian J Ophthalmol Date: 2015-03 Impact factor: 1.848