Literature DB >> 1190509

Separation of X- and Y-bearing human spermatozoa with the sephadex gel-filtration method.

O Steeno, A Adimoelja, J Steeno.   

Abstract

By means of the Sephadex gel filtration method we were able to separate a fraction rich in X-bearing human spermatozoa, in contrast with the method of Ericsson et al. (1973), which allows to obtain a fraction rich in Y-bearing sperm. The method, which is simple and not time-consuming, may allow control of sex in cases of expressed sex desire in off-spring.

Entities:  

Keywords:  Biology; Clinical Research; Genitalia; Germ Cells; Human Volunteers; Physiology; Research Methodology; Spermatozoa--analysis; Urogenital System

Mesh：

Year:  1975        PMID： 1190509     DOI： 10.1111/j.1439-0272.1975.tb01234.x

Source DB:  PubMed          Journal:  Andrologia        ISSN： 0303-4569            Impact factor:   2.775


  8 in total

1.  When not to choose: a case study.

Authors:  Betty B Hoskins; Helen Bequaert Holmes
Journal:  J Med Humanit Bioeth       Date:  1985 Spring-Summer

2.  Studies on frequency of Y chromatin in human sperm.

Authors:  E Schwinger; J Ites; B Korte
Journal:  Hum Genet       Date:  1976-12-15       Impact factor: 4.132

3.  Tentative identification of sex-specific antibodies and their application for screening bovine sperm proteins for sex-specificity.

Authors:  Wu-Cai Yang; Lei Sang; Yao Xiao; Hua-Lin Zhang; Ke-Qiong Tang; Li-Guo Yang
Journal:  Mol Biol Rep       Date:  2013-11-12       Impact factor: 2.316

4.  Separating X-bearing human spermatozoa through a discontinuous Percoll density gradient proved to be inefficient by double-label fluorescent in situ hybridization.

Authors:  S P Lin; R K Lee; Y J Tsai; Y M Hwu; M H Lin
Journal:  J Assist Reprod Genet       Date:  1998-10       Impact factor: 3.412

5.  Spermatozoa selection by the swim-up procedure and two-layer percoll gradient centrifugation.

Authors:  Y Evliyaoğlu; U Ciftçi; N Bozdemir
Journal:  Int Urol Nephrol       Date:  1996       Impact factor: 2.370

Review 6.  An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm.

Authors:  Shiv Kumar Yadav; Dharmendra Kumar Gangwar; Jarnail Singh; Chiranjeev Kumar Tikadar; V Vinoth Khanna; Sudha Saini; Sunny Dholpuria; Prabhat Palta; Radhey Shyam Manik; Manoj Kumar Singh; Suresh Kumar Singla
Journal:  Vet World       Date:  2017-05-09

7.  Effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine.

Authors:  Nidhi P Raval; Tejas M Shah; Linz-Buoy George; Chaitanya G Joshi
Journal:  Vet World       Date:  2019-08-23

Review 8.  Bovine semen sexing: Sperm membrane proteomics as candidates for immunological selection of X- and Y-chromosome-bearing sperm.

Authors:  Joana Quelhas; Joana Santiago; Bárbara Matos; António Rocha; Graça Lopes; Margarida Fardilha
Journal:  Vet Med Sci       Date:  2021-05-26
  8 in total

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