| Literature DB >> 119034 |
N Rastogi, T A Venkitasubramanian.
Abstract
Cell wall-deficient forms of Mycobacterium smegmatis were produced in growth medium containing D-cycloserine and horse serum. These cells were transformed into protoplasts with EDTA and lysozyme. Subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).Entities:
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Year: 1979 PMID： 119034 DOI： 10.1099/00221287-115-2-517
Source DB: PubMed Journal: J Gen Microbiol ISSN： 0022-1287