Er:YAG laser effects on enamel occlusal fissures: an in vitro study.

This study evaluated by scanning electron microscopy (SEM) the morphological changes in occlusal fissure enamel, of permanent models, irradiated by Er:YAG laser using contact and noncontact fiberoptics in vitro. Previous studies have demonstrated the efficacy of Er:YAG laser for dental hard tissue removal and cavity preparation. The treatment of occlusal fissures in noncarious permanent human molars (n = 9) was carried out with Er:YAG laser (KEY Laser II) using handpiece no. 2051, noncontact, focused (12 mm), water spray-cooled, pulse energy 200 mJ, and frequency 2 Hz (group 1), and handpiece no. 2055 with a quartz fiberoptic 50/10, in contact, air cooled, pulse energy setting of 350 mJ and frequency 2 Hz (group 2) and 400 mJ/2 Hz (group 3). The specimens were sectioned, dehydrated in a graded series of aqueous ethanol, dried, and sputtering with gold. Morphological change analysis on occlusal fissures was performed by SEM. Group 1 showed removal of fissure debris and predominantly enamel etching-like patterns, and groups 2 and 3 showed irregular edges, melting, and recrystallization of fissure enamel, with a lava-like structure and bubble-like voids. The results of this in vitro study suggest that the irradiation of fissures by Er:YAG laser using a fiberoptics (contact and air cooled) produced melting and recrystallization of fissures enamel. Further studies are required with different energy parameters and water cooling to evaluate the thermal effects on teeth.