| Literature DB >> 11900485 |
Renu Wadhwa1, Tomoko Yaguchi, Md K Hasan, Youji Mitsui, Roger R Reddel, Sunil C Kaul.
Abstract
Hsp70 family member mot-2/mthsp70/GRP75/PBP74 was shown to bind to the tumor suppressor protein p53. In this study, by in vivo coimmunoprecipitation of mot-2 with p53 and its deletion mutants, the mot-2 binding site of p53 was mapped to its C-terminal amino acid residues 312-352, a region of p53 that includes its cytoplasmic sequestration domain. These data demonstrate that cytoplasmic sequestration and inactivation of p53 by mot-2 occurs by its binding to the cytoplasmic sequestration domain. Therefore, perturbation of mot-p53 interactions can be employed to abrogate cytoplasmic retention of wild-type p53 in tumors. Copyright 2002 Elsevier Science (USA).Entities:
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Year: 2002 PMID： 11900485 DOI： 10.1006/excr.2002.5468
Source DB: PubMed Journal: Exp Cell Res ISSN： 0014-4827 Impact factor: 3.905