Literature DB >> 11898341

Production, purification and characterization of intracellular alanylaminopeptidase of Pseudomonas sp.

U Jankiewicz1, W Bielawski.   

Abstract

The soil bacterium Pseudomonas sp. was found to synthesize an aminopeptidase that prefers Ala-beta-naphtylamide as substrate. The enzyme was purified 660-fold by ammonium sulfate fractionation, preparative electrophoresis, ion exchange chromatography on Protein-Pak Q 8 HR and molecular sieving chromatography on Zorbax SE-250. When purified to homogeneity, the enzyme was shown to be a monomeric protein with a molar mass of 65 kDa; it showed a maximum activity at pH 7.5 and 45 degrees C.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 11898341     DOI： 10.1007/bf02817995

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  25 in total

1.  Characterization of a solvent resistant and thermostable aminopeptidase from the hyperthermophillic bacterium, Aquifex aeolicus.

Authors: 
Journal:  Enzyme Microb Technol       Date:  2000-07-01       Impact factor: 3.493

2.  Nucleotide sequence of the pepN gene encoding aminopeptidase N of Escherichia coli.

Authors:  M Foglino; S Gharbi; A Lazdunski
Journal:  Gene       Date:  1986       Impact factor: 3.688

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Regulation of Escherichia coli K10 aminoendopeptidase synthesis. Effects of mutations involved in the regulation of alkaline phosphatase.

Authors:  A Lazdunski; C Pellissier; C Lazdunski
Journal:  Eur J Biochem       Date:  1975-12-15

Review 5.  Aminopeptidases: structure and function.

Authors:  A Taylor
Journal:  FASEB J       Date:  1993-02-01       Impact factor: 5.191

6.  A comparative study of bacterial alanine aminohydrolases.

Authors:  F J Behal; J D Folds
Journal:  Biochem Biophys Res Commun       Date:  1967-05-05       Impact factor: 3.575

7.  Purification and characterization of a lysine-p-nitroanilide hydrolase, a broad specificity aminopeptidase, from the cytoplasm of Lactococcus lactis subsp. cremoris AM2.

Authors:  M McDonnell; P Bouchier; R J Fitzgerald; G O'Cuinn
Journal:  J Dairy Res       Date:  1999-05       Impact factor: 1.904

8.  Cloning, DNA sequence analysis and partial characterization of pepN, a lysyl aminopeptidase from Lactobacillus delbrückii ssp. lactis DSM7290.

Authors:  J R Klein; U Klein; M Schad; R Plapp
Journal:  Eur J Biochem       Date:  1993-10-01

9.  Intracellular activation of albomycin in Escherichia coli and Salmonella typhimurium.

Authors:  V Braun; K Günthner; K Hantke; L Zimmermann
Journal:  J Bacteriol       Date:  1983-10       Impact factor: 3.490

10.  Purification and characterization of ascamycin-hydrolysing aminopeptidase from Xanthomonas citri.

Authors:  H Osada; K Isono
Journal:  Biochem J       Date:  1986-01-15       Impact factor: 3.857
View more
  1 in total

1.  Regulation of the activity of intracellular alanylaminopeptidase synthesized by Pseudomonas sp.

Authors:  U Jankiewicz; W Bielawski
Journal:  Folia Microbiol (Praha)       Date:  2002       Impact factor: 2.099
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.