Literature DB >> 11898335

Novel, thermostable family-13-like glycoside hydrolase from Methanococcus jannaschii.

J W Kim1, H A Terc, L O Flowers, M Whiteley, T L Peeples.   

Abstract

A novel glycoside hydrolase from the hyperthermophilic archaeon Methanococcus jannaschii has been cloned into Escherichia coli. Extremely thermoactive and thermostable amylolytic activity was confirmed in partially purified enzyme solution. This enzyme exhibited a temperature optimum of 100 degrees C and a pH optimum pH 5.0-8.0. Hydrolysis of large 1,6-alpha- and 1,4-alpha-linked polysaccharides yielded glucose polymers of 1-7 units. Incubation with amylose displayed the highest activity. The catalyst was activated and stabilized by Ca2+ and exhibited extreme thermostability at 100 degrees C with a half-life of 78 h.

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Year:  2001        PMID： 11898335     DOI： 10.1007/bf02817989

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  17 in total

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2.  Characterization of Amylolytic Enzyme Activities Associated with the Hyperthermophilic Archaebacterium Pyrococcus furiosus.

Authors:  S H Brown; H R Costantino; R M Kelly
Journal:  Appl Environ Microbiol       Date:  1990-07       Impact factor: 4.792

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Authors:  E L Sonnhammer; S R Eddy; R Durbin
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4.  Cloning, sequencing, and expression of the gene encoding amylopullulanase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme.

Authors:  G Dong; C Vieille; J G Zeikus
Journal:  Appl Environ Microbiol       Date:  1997-09       Impact factor: 4.792

5.  Improved tools for biological sequence comparison.

Authors:  W R Pearson; D J Lipman
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205

6.  Molecular genetic analysis of the pullulanase B gene of Bacillus acidopullulyticus.

Authors:  A P Kelly; B Diderichsen; S Jorgensen; D J McConnell
Journal:  FEMS Microbiol Lett       Date:  1994-01-01       Impact factor: 2.742

7.  A new generation of information retrieval tools for biologists: the example of the ExPASy WWW server.

Authors:  R D Appel; A Bairoch; D F Hochstrasser
Journal:  Trends Biochem Sci       Date:  1994-06       Impact factor: 13.807

8.  Sequence of archaeal Methanococcus jannaschii alpha-amylase contains features of families 13 and 57 of glycosyl hydrolases: a trace of their common ancestor?

Authors:  S Janecek
Journal:  Folia Microbiol (Praha)       Date:  1998       Impact factor: 2.099

9.  Three dimensional structure of porcine pancreatic alpha-amylase at 2.9 A resolution. Role of calcium in structure and activity.

Authors:  G Buisson; E Duée; R Haser; F Payan
Journal:  EMBO J       Date:  1987-12-20       Impact factor: 11.598

10.  Genome sequences and great expectations.

Authors:  I Iliopoulos; S Tsoka; M A Andrade; P Janssen; B Audit; A Tramontano; A Valencia; C Leroy; C Sander; C A Ouzounis
Journal:  Genome Biol       Date:  2000-12-29       Impact factor: 13.583
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  1 in total

1.  Biochemical confirmation and characterization of the family-57-like alpha-amylase of Methanococcus jannaschii.

Authors:  J W Kim; L O Flowers; M Whiteley; T L Peeples
Journal:  Folia Microbiol (Praha)       Date:  2001       Impact factor: 2.099
  1 in total

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