Literature DB >> 11894772

Ultrastructure of guinea pig stria vascularis processed by rapid freezing and freeze substitution.

H Kawaguchi1, T Arima, T Uemura.   

Abstract

The rapid-freezing and freeze-substitution method fixes a specimen as if it were prepared before excision. We used this method to study the stria vascularis of guinea pigs using electron microscopy. Findings were essentially the same as those obtained with conventional chemical fixation, although freeze substitution made it possible to observe the membrane structures in a smoother and more linear manner. This method seems to be the procedure of choice for studying the instantaneous movement and behavior of cells.

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Year:  1994        PMID： 11894772     DOI： 10.1007/bf02565216

Source DB:  PubMed          Journal:  Eur Arch Otorhinolaryngol        ISSN： 0937-4477            Impact factor:   2.503


  10 in total

1.  Postnatal development of endocochlear potential and stria vascularis in the cat.

Authors:  C Fernández; R Hinojosa
Journal:  Acta Otolaryngol       Date:  1974 Sep-Oct       Impact factor: 1.494

2.  The preservation of tissue fine structure during rapid freezing.

Authors:  D C Pease
Journal:  J Ultrastruct Res       Date:  1967-11

3.  Ultrastructural observations on chick bone processed by quick-freezing and freeze-substitution.

Authors:  T Akisaka; G P Subita; Y Shigenaga
Journal:  Cell Tissue Res       Date:  1987-03       Impact factor: 5.249

4.  A copper block method for freezing non-cryoprotected tissue to produce ice-crystal-free regions for electron microscopy. I. Evaluation using freeze-substitution.

Authors:  G P Dempsey; S Bullivant
Journal:  J Microsc       Date:  1976-04       Impact factor: 1.758

5.  Extraction of membrane lipids during fixation, dehydration and embedding of Acholeplasma laidlawii-cells for electron microscopy.

Authors:  C Weibull; A Christiansson; E Carlemalm
Journal:  J Microsc       Date:  1983-02       Impact factor: 1.758

6.  An ultrastructural study of nerve and glial cells by freeze-substitution.

Authors:  N Hirokawa; T Kirino
Journal:  J Neurocytol       Date:  1980-04

7.  Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release.

Authors:  J E Heuser; T S Reese; M J Dennis; Y Jan; L Jan; L Evans
Journal:  J Cell Biol       Date:  1979-05       Impact factor: 10.539

8.  Rapid-freezing and malachite green-acrolein-osmium tetroxide freeze-substitution fixation improve visualization of extracellular lipids in rat incisor pre-dentin and dentin.

Authors:  M Goldberg; F Escaig
Journal:  J Histochem Cytochem       Date:  1987-04       Impact factor: 2.479

9.  The fine structure of sublingual gland acinar cells of the Mongolian gerbil, Meriones unguiculatus, processed by rapid freezing followed by freeze-substitution fixation.

Authors:  M Ichikawa; A Ichikawa
Journal:  Cell Tissue Res       Date:  1987-11       Impact factor: 5.249

10.  Ultrastructural localization of K+-dependent, ouabain-sensitive NPPase (Na-K-ATPase) in the guinea pig inner ear.

Authors:  K Mees
Journal:  Acta Otolaryngol       Date:  1983 Mar-Apr       Impact factor: 1.494
  10 in total

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