UNLABELLED: Exercise is associated with intensity-dependent immune disturbances. Leukocyte mitochondrial alterations and apoptosis may contribute to this phenomenon. PURPOSE: To investigate the effects of different intensities of aerobic exercise (AE) on leukocyte mitochondrial transmembrane potential (MTP) and the propensity of apoptosis. METHODS: Blood samples were collected from 12 subjects who performed AE for 3 consecutive days (35% maximal oxygen consumption (VO2max)). Leukocyte MTP and apoptosis were measured by flow cytometry. The subjects performed two additional sessions of AE of higher intensities (60% and 85% VO2max) with an intervening 4-wk washout period. The measurements were repeated during each session. RESULTS: Leukocyte MTP declined during daily, repetitive AE at an intensity of 60% and 85% VO2max. Similar changes were not found during a more moderate AE (35% VO2max). Leukocytes increased their propensity of apoptosis a period (3-5 d) after the start of the AE. CONCLUSION: High-intensity AE has accumulative effects on the mitochondrial energization status and vitality of peripheral blood leukocytes. Leukocyte MTP is a potentially applicable indicator for monitoring immune distress due to overtraining.
UNLABELLED: Exercise is associated with intensity-dependent immune disturbances. Leukocyte mitochondrial alterations and apoptosis may contribute to this phenomenon. PURPOSE: To investigate the effects of different intensities of aerobic exercise (AE) on leukocyte mitochondrial transmembrane potential (MTP) and the propensity of apoptosis. METHODS: Blood samples were collected from 12 subjects who performed AE for 3 consecutive days (35% maximal oxygen consumption (VO2max)). Leukocyte MTP and apoptosis were measured by flow cytometry. The subjects performed two additional sessions of AE of higher intensities (60% and 85% VO2max) with an intervening 4-wk washout period. The measurements were repeated during each session. RESULTS: Leukocyte MTP declined during daily, repetitive AE at an intensity of 60% and 85% VO2max. Similar changes were not found during a more moderate AE (35% VO2max). Leukocytes increased their propensity of apoptosis a period (3-5 d) after the start of the AE. CONCLUSION: High-intensity AE has accumulative effects on the mitochondrial energization status and vitality of peripheral blood leukocytes. Leukocyte MTP is a potentially applicable indicator for monitoring immune distress due to overtraining.
Authors: James W Navalta; Refaat Mohamed; Ayman El-Baz; Brian K McFarlin; T Scott Lyons Journal: Eur J Appl Physiol Date: 2010-05-20 Impact factor: 3.078
Authors: Adriana Cristina Levada-Pires; C E R Fonseca; E Hatanaka; T Alba-Loureiro; A D'Angelo; F B Velhote; R Curi; T C Pithon-Curi Journal: Eur J Appl Physiol Date: 2010-02-09 Impact factor: 3.078
Authors: Maria Fernanda Cury-Boaventura; Adriana C Levada-Pires; Alessandra Folador; Renata Gorjão; Tatiana C Alba-Loureiro; Sandro M Hirabara; Fabiano P Peres; Paulo R S Silva; Rui Curi; Tania C Pithon-Curi Journal: Eur J Appl Physiol Date: 2008-03-05 Impact factor: 3.078