| Literature DB >> 11880398 |
Terri H Hardwick1, Brian Plikaytis, Pamela K Cassiday, Gary Cage, Mark S Peppler, Deborah Shea, David Boxrud, Gary N Sanden.
Abstract
The intra- and interlaboratory variabilities of the molecular size measurements of each DNA fragment contributing to three pulsed-field gel electrophoresis (PFGE) profiles were assessed, as were the reproducibilities of the entire PFGE profiles for three Bordetella pertussis strains. The major source of variability within a laboratory occurred between subcultures rather than within gels or between gels. Each PFGE profile was generated reproducibly and was objectively defined by the molecular sizes of its composite fragments. A strain or profile most suitable for use as an internal reference standard was identified.Mesh:
Substances:
Year: 2002 PMID: 11880398 PMCID: PMC120225 DOI: 10.1128/JCM.40.3.811-816.2002
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948