OBJECTIVES: To study single nucleotide polymorphisms to investigate the possibility that urokinase is involved in the formation of urolithiasis, which, although lacking in genetic evidence, has been previously proposed. METHODS: A total of 153 patients with recurrent calcium stones and 105 controls were studied. Polymerase chain reaction-based restriction analysis was used to identify the C/T polymorphism of the urokinase gene, which is mapped on the 3'-untranslated region (3'-UTR) on chromosome 10. RESULTS: A significant difference was found in the distribution of the urokinase gene 3'-UTR C/T polymorphism frequency between patients with stones and normal controls (P <0.05). The odds ratio for the risk of the "T" allele in patients with stones was 3.088 (95% confidence interval 1.06 to 8.99). CONCLUSIONS: The results of our study demonstrate that the urokinase gene 3'-UTR "T" allele is associated with calcium stone disease. Individuals possessing the "T" allele have a higher incidence of calcium oxalate stone disease. The results of this study provide genetic evidence that the urokinase gene may play a role in stone formation.
OBJECTIVES: To study single nucleotide polymorphisms to investigate the possibility that urokinase is involved in the formation of urolithiasis, which, although lacking in genetic evidence, has been previously proposed. METHODS: A total of 153 patients with recurrent calcium stones and 105 controls were studied. Polymerase chain reaction-based restriction analysis was used to identify the C/T polymorphism of the urokinase gene, which is mapped on the 3'-untranslated region (3'-UTR) on chromosome 10. RESULTS: A significant difference was found in the distribution of the urokinase gene 3'-UTR C/T polymorphism frequency between patients with stones and normal controls (P <0.05). The odds ratio for the risk of the "T" allele in patients with stones was 3.088 (95% confidence interval 1.06 to 8.99). CONCLUSIONS: The results of our study demonstrate that the urokinase gene 3'-UTR "T" allele is associated with calciumstone disease. Individuals possessing the "T" allele have a higher incidence of calciumoxalate stone disease. The results of this study provide genetic evidence that the urokinase gene may play a role in stone formation.
Authors: Serdar Aykan; Murat Tuken; Sezgin Gunes; Yigit Akin; Murat Ozturk; Serkan Seyhan; Emrah Yuruk; Mustafa Zafer Temiz; Ali Faik Yılmaz; Daniel P Nguyen Journal: Urolithiasis Date: 2015-08-15 Impact factor: 3.436
Authors: Prasun J Mishra; Rita Humeniuk; Pravin J Mishra; Giuseppe S A Longo-Sorbello; Debabrata Banerjee; Joseph R Bertino Journal: Proc Natl Acad Sci U S A Date: 2007-08-08 Impact factor: 11.205