Literature DB >> 11875432

Production of a freeze-thaw-stable potato starch by antisense inhibition of three starch synthase genes.

Stephen A Jobling1, Roger J Westcott, Akash Tayal, Roger Jeffcoat, Gerhard P Schwall.   

Abstract

The use of unmodified starches in frozen foods is severely limited by the undesirable textural changes that occur after freezing and thawing. Retrogradation of glucan chains leads to syneresis, a separation of the starch gel and water phases. Stabilization of the starch structure is normally achieved by chemical modification to prevent these changes from occurring. We have now created a freeze-thaw-stable potato starch by alteration of starch composition and structure by genetic modification. An amylose-free starch with short-chain amylopectin was produced by simultaneous antisense downregulation of three starch synthase genes. This starch is extremely freeze-thaw-stable and shows no syneresis even after five freeze-thaw cycles. The use of this starch has potential for environmental and consumer benefits because its production requires no chemical modification.

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Year:  2002        PMID: 11875432     DOI: 10.1038/nbt0302-295

Source DB:  PubMed          Journal:  Nat Biotechnol        ISSN: 1087-0156            Impact factor:   54.908


  18 in total

1.  Diurnal changes in the transcriptome encoding enzymes of starch metabolism provide evidence for both transcriptional and posttranscriptional regulation of starch metabolism in Arabidopsis leaves.

Authors:  Steven M Smith; Daniel C Fulton; Tansy Chia; David Thorneycroft; Andrew Chapple; Hannah Dunstan; Christopher Hylton; Samuel C Zeeman; Alison M Smith
Journal:  Plant Physiol       Date:  2004-09-03       Impact factor: 8.340

2.  Accumulation of multiple-repeat starch-binding domains (SBD2-SBD5) does not reduce amylose content of potato starch granules.

Authors:  Farhad Nazarian Firouzabadi; Jean-Paul Vincken; Qin Ji; Luc C J M Suurs; Alain Buléon; Richard G F Visser
Journal:  Planta       Date:  2007-03       Impact factor: 4.116

3.  Expression of an amylosucrase gene in potato results in larger starch granules with novel properties.

Authors:  Xing-Feng Huang; Farhad Nazarian-Firouzabadi; Jean-Paul Vincken; Qin Ji; Richard G F Visser; Luisa M Trindade
Journal:  Planta       Date:  2014-06-04       Impact factor: 4.116

4.  Expression of multiple resistance genes enhances tolerance to environmental stressors in transgenic poplar (Populus × euramericana 'Guariento').

Authors:  Xiaohua Su; Yanguang Chu; Huan Li; Yingjie Hou; Bingyu Zhang; Qinjun Huang; Zanmin Hu; Rongfeng Huang; Yingchuan Tian
Journal:  PLoS One       Date:  2011-09-09       Impact factor: 3.240

5.  Expression of alternansucrase in potato plants.

Authors:  Géraldine A Kok-Jacon; Jean-Paul Vincken; Luc C J M Suurs; Denong Wang; Shaoyi Liu; Richard G F Visser
Journal:  Biotechnol Lett       Date:  2007-03-23       Impact factor: 2.461

6.  Overexpression of STARCH BRANCHING ENZYME II increases short-chain branching of amylopectin and alters the physicochemical properties of starch from potato tuber.

Authors:  David A Brummell; Lyn M Watson; Jun Zhou; Marian J McKenzie; Ian C Hallett; Lyall Simmons; Margaret Carpenter; Gail M Timmerman-Vaughan
Journal:  BMC Biotechnol       Date:  2015-04-29       Impact factor: 2.563

Review 7.  Formation of starch in plant cells.

Authors:  Barbara Pfister; Samuel C Zeeman
Journal:  Cell Mol Life Sci       Date:  2016-05-11       Impact factor: 9.261

8.  Biological Implications in Cassava for the Production of Amylose-Free Starch: Impact on Root Yield and Related Traits.

Authors:  Amanda Karlström; Fernando Calle; Sandra Salazar; Nelson Morante; Dominique Dufour; Hernán Ceballos
Journal:  Front Plant Sci       Date:  2016-05-20       Impact factor: 5.753

Review 9.  Underpinning Starch Biology with in vitro Studies on Carbohydrate-Active Enzymes and Biosynthetic Glycomaterials.

Authors:  Ellis C O'Neill; Robert A Field
Journal:  Front Bioeng Biotechnol       Date:  2015-09-07

10.  Analysis of the functional interaction of Arabidopsis starch synthase and branching enzyme isoforms reveals that the cooperative action of SSI and BEs results in glucans with polymodal chain length distribution similar to amylopectin.

Authors:  Henrike Brust; Tanja Lehmann; Christophe D'Hulst; Joerg Fettke
Journal:  PLoS One       Date:  2014-07-11       Impact factor: 3.240

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