Literature DB >> 11874903

PrP(Sc) is not detected in peripheral blood leukocytes of scrapie-infected sheep: determining the limit of sensitivity by immunohistochemistry.

Lynn M Herrmann1, Timothy V Baszler, Donald P Knowles, William P Cheevers.   

Abstract

Peripheral blood leukocytes (PBLs) from scrapie-infected sheep were evaluated for the presence of PrP(Sc) by using dissociated retropharyngeal lymph node (DRLN) cells and immunohistochemistry (IHC). PrP(Sc)-positive cells were detected in 2.05% +/- 0.28% of 3 x 10(6) DRLN cells, but were not detected in 3 x 10(6) PBLs from scrapie-infected sheep. Titration of DRLN cells mixed with PBLs showed that IHC detects a minimum of 0.00205% or 60 PrP(Sc)-positive cells in 3 x 10(6) PBLs.

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Year:  2002        PMID： 11874903      PMCID： PMC119962          DOI： 10.1128/cdli.9.2.499-502.2002

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  14 in total

1.  Binding of disease-associated prion protein to plasminogen.

Authors:  M B Fischer; C Roeckl; P Parizek; H P Schwarz; A Aguzzi
Journal:  Nature       Date:  2000-11-23       Impact factor: 49.962

2.  Sites of prion protein accumulation in scrapie-infected mouse spleen revealed by immuno-electron microscopy.

Authors:  M Jeffrey; G McGovern; C M Goodsir; K L Brown; M E Bruce
Journal:  J Pathol       Date:  2000-07       Impact factor: 7.996

3.  Pathogenesis of scrapie virus infection in the mouse.

Authors:  C M Eklund; R C Kennedy; W J Hadlow
Journal:  J Infect Dis       Date:  1967-02       Impact factor: 5.226

4.  Identification of a protein that purifies with the scrapie prion.

Authors:  D C Bolton; M P McKinley; S B Prusiner
Journal:  Science       Date:  1982-12-24       Impact factor: 47.728

5.  Distribution of prion protein in the ileal Peyer's patch of scrapie-free lambs and lambs naturally and experimentally exposed to the scrapie agent.

Authors:  Ragna Heggebø; Charles McL Press; Gjermund Gunnes; Kai Inge Lie; Michael A Tranulis; Martha Ulvund; Martin H Groschup; Thor Landsverk
Journal:  J Gen Virol       Date:  2000-09       Impact factor: 3.891

6.  Preclinical diagnosis of scrapie by immunohistochemistry of third eyelid lymphoid tissue.

Authors:  K I O'Rourke; T V Baszler; T E Besser; J M Miller; R C Cutlip; G A Wells; S J Ryder; S M Parish; A N Hamir; N E Cockett; A Jenny; D P Knowles
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948

7.  Cellular and sub-cellular localisation of PrP in the lymphoreticular system of mice and sheep.

Authors:  M Jeffrey; G McGovern; S Martin; C M Goodsir; K L Brown
Journal:  Arch Virol Suppl       Date:  2000

8.  Use of capillary electrophoresis and fluorescent labeled peptides to detect the abnormal prion protein in the blood of animals that are infected with a transmissible spongiform encephalopathy.

Authors:  M J Schmerr; A L Jenny; M S Bulgin; J M Miller; A N Hamir; R C Cutlip; K R Goodwin
Journal:  J Chromatogr A       Date:  1999-08-20       Impact factor: 4.759

9.  Further studies of blood infectivity in an experimental model of transmissible spongiform encephalopathy, with an explanation of why blood components do not transmit Creutzfeldt-Jakob disease in humans.

Authors:  P Brown; L Cervenáková; L M McShane; P Barber; R Rubenstein; W N Drohan
Journal:  Transfusion       Date:  1999 Nov-Dec       Impact factor: 3.157

10.  Natural infection of Suffolk sheep with scrapie virus.

Authors:  W J Hadlow; R C Kennedy; R E Race
Journal:  J Infect Dis       Date:  1982-11       Impact factor: 5.226
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  5 in total

1.  CD21-positive follicular dendritic cells: A possible source of PrPSc in lymph node macrophages of scrapie-infected sheep.

Authors:  Lynn M Herrmann; William P Cheevers; William C Davis; Donald P Knowles; Katherine I O'Rourke
Journal:  Am J Pathol       Date:  2003-04       Impact factor: 4.307

2.  Modification of blood cell PrP epitope exposure during prion disease.

Authors:  Alana M Thackray; Stephen J Ryder; Raymond Bujdoso
Journal:  Biochem J       Date:  2005-09-01       Impact factor: 3.857

3.  Detection of PrPsc in blood from sheep infected with the scrapie and bovine spongiform encephalopathy agents.

Authors:  L A Terry; L Howells; J Hawthorn; J C Edwards; S J Moore; S J Bellworthy; H Simmons; S Lizano; L Estey; V Leathers; S J Everest
Journal:  J Virol       Date:  2009-09-09       Impact factor: 5.103

4.  Accumulation and dissemination of prion protein in experimental sheep scrapie in the natural host.

Authors:  Stephen J Ryder; Glenda E Dexter; Lindsay Heasman; Richard Warner; S Jo Moore
Journal:  BMC Vet Res       Date:  2009-02-25       Impact factor: 2.741

Review 5.  Detection of Pathognomonic Biomarker PrPSc and the Contribution of Cell Free-Amplification Techniques to the Diagnosis of Prion Diseases.

Authors:  Hasier Eraña; Jorge M Charco; Ezequiel González-Miranda; Sandra García-Martínez; Rafael López-Moreno; Miguel A Pérez-Castro; Carlos M Díaz-Domínguez; Adrián García-Salvador; Joaquín Castilla
Journal:  Biomolecules       Date:  2020-03-19
  5 in total

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