Literature DB >> 11874883

Sensitive microplate assay for detection of bactericidal antibodies to Vibrio cholerae O139.

Stephen R Attridge1, Camilla Johansson, Dang D Trach, Firdausi Qadri, Ann-Mari Svennerholm.   

Abstract

A microplate assay for the detection of bactericidal antibodies to Vibrio cholerae O139 is described. The assay is sensitive, highly reproducible, specific, and convenient to perform. It has been used to demonstrate the induction of serum bactericidal antibodies in Vietnamese recipients of an oral, inactivated, bivalent O1/O139 vaccine, as well as in Bangladeshi patients with O139 disease. In both study groups there was a significant inverse correlation between the preexposure level of antibodies in serum and the magnitude of the subsequent bactericidal response. Although infection generated stronger responses than vaccination, the proportion of responders was similar among individuals with low background titers.

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Year:  2002        PMID: 11874883      PMCID: PMC119965          DOI: 10.1128/cdli.9.2.383-387.2002

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN: 1071-412X


  15 in total

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Authors:  M Jertborn; A M Svennerholm; J Holmgren
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Journal:  Bull World Health Organ       Date:  2002       Impact factor: 9.408

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5.  Vibriocidal antibody responses in North American volunteers exposed to wild-type or vaccine Vibrio cholerae O139: specificity and relevance to immunity.

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Journal:  Clin Diagn Lab Immunol       Date:  1997-05

6.  Large epidemic of cholera-like disease in Bangladesh caused by Vibrio cholerae O139 synonym Bengal. Cholera Working Group, International Centre for Diarrhoeal Diseases Research, Bangladesh.

Authors: 
Journal:  Lancet       Date:  1993-08-14       Impact factor: 79.321

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Authors:  S R Attridge; F Qadri; M J Albert; P A Manning
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8.  Clinical and immunologic characteristics of Vibrio cholerae O139 Bengal infection in North American volunteers.

Authors:  J G Morris; G E Losonsky; J A Johnson; C O Tacket; J P Nataro; P Panigrahi; M M Levin
Journal:  J Infect Dis       Date:  1995-04       Impact factor: 5.226

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