| Literature DB >> 11869798 |
Jin Yin1, Luke Tomycz, Gary Bonner, Daniel I C Wang.
Abstract
An assay for the quantification of collagen-like polymer (CLP) in Escherichia coli cells utilizing the specific reaction between collagenase and CLP is presented. It involves thermal treatment to precipitate non-CLP proteins, digestion of CLP by collagenase and detection of the absorbance of the liberated amino acids and peptides from CLP by a ninhydrin-based method. CLP concentration is determined from the absorbance measurement.Entities:
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Year: 2002 PMID： 11869798 DOI： 10.1016/s0167-7012(01)00372-4
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363