J Liu1, L Tang, X Liu, X Zhang, H Gu. 1. Department of Ophthalmology, Xiangya Second Hospital, Certral South University, Changsha 410011, China.
Abstract
OBJECTIVE: To investigate the expression of multidrug resistance gene (MDR1) and multidrug associated protein (MRP) gene in the retinoblastoma (Rb) cell line HXO-RB(44), and study the mechanism of multidrug resistance (MDR) phenotype occurring in retinoblastoma. METHODS: Reverse transcriptase polymerase chain reaction (RT-PCR) was employed to detect the transcription of MDR1 and MRP gene in the HXO-RB(44) cell line, and immunohistochemical technique was used to detect their protein products P-glycoprotein and multidrug resistance associated protein (P190). RESULT: Both MDR1 and MRP genes were expressed in HXO-RB(44) cell line, as well as P-glycoprotein and P190 were over-expressed (96% - 97%) in this cell line. CONCLUSION: Over-expression of MDR1 and MRP genes is significantly involved in the mechanism of MDR phenotype in retinoblastoma.
OBJECTIVE: To investigate the expression of multidrug resistance gene (MDR1) and multidrug associated protein (MRP) gene in the retinoblastoma (Rb) cell line HXO-RB(44), and study the mechanism of multidrug resistance (MDR) phenotype occurring in retinoblastoma. METHODS: Reverse transcriptase polymerase chain reaction (RT-PCR) was employed to detect the transcription of MDR1 and MRP gene in the HXO-RB(44) cell line, and immunohistochemical technique was used to detect their protein products P-glycoprotein and multidrug resistance associated protein (P190). RESULT: Both MDR1 and MRP genes were expressed in HXO-RB(44) cell line, as well as P-glycoprotein and P190 were over-expressed (96% - 97%) in this cell line. CONCLUSION: Over-expression of MDR1 and MRP genes is significantly involved in the mechanism of MDR phenotype in retinoblastoma.
Authors: S Krishnakumar; K Mallikarjuna; N Desai; A Muthialu; N Venkatesan; A Sundaram; V Khetan; M P Shanmugam Journal: Br J Ophthalmol Date: 2004-12 Impact factor: 4.638