L Hao1, M Liu, X Liu, X Chen, Y Tang, Z Li. 1. National Reference Laboratory for Reproductive Health Research, Beijing Medical University, Beijing, 100083, China.
Abstract
OBJECTIVE: To study relationship between serum and red blood cell (RBC) folate, serum vitamin B(12) (VB(12)), total plasma homocysteine (tHcy), thermolabile genotypes, and activity of methylene-tetrahydrofolate reductase (MTHFR). METHODS: Thirty-four non-pregnant and healthy mothers who gave birth to babies with neural tube defects (NTDs) and 42 mothers to normal babies previously were chosen. Plasma tHcy, RBC and serum folate, serum VB(12), activity of MTHFR and thermolabile genotypes were determined for all of them. RESULTS: There was no significant difference in plasma tHcy, RBC and serun folate between case and control groups. Serum VB(12) was significantly lower in mothers of babies with NTDs than in those with normal ones. Plasma tHcy correlated with RBC folate inversely (P < 0.05). Mean plasma tHcy for thermolabile homozygous mutation of MTHFR was significantly higher than that for either heterozygous mutation or wild homozygous mutation (P < 0.001). There was no significant difference in serum folate between groups with varied MTHFR genotypes. Plasma tHcy was slightly greater in those with lower serum folate than in those with higher one, and correlated inversely with activity of MTHFR. CONCLUSIONS: Increase in plasma tHcy correlates with homozygous mutation of thermolabile MTHFR and decrease in activity of MTHFR. Higher level of folate in the body can interfere the relationship between plasma tHcy and activity of MTHFR.
OBJECTIVE: To study relationship between serum and red blood cell (RBC) folate, serum vitamin B(12) (VB(12)), total plasma homocysteine (tHcy), thermolabile genotypes, and activity of methylene-tetrahydrofolate reductase (MTHFR). METHODS: Thirty-four non-pregnant and healthy mothers who gave birth to babies with neural tube defects (NTDs) and 42 mothers to normal babies previously were chosen. Plasma tHcy, RBC and serum folate, serum VB(12), activity of MTHFR and thermolabile genotypes were determined for all of them. RESULTS: There was no significant difference in plasma tHcy, RBC and serun folate between case and control groups. Serum VB(12) was significantly lower in mothers of babies with NTDs than in those with normal ones. Plasma tHcy correlated with RBC folate inversely (P < 0.05). Mean plasma tHcy for thermolabile homozygous mutation of MTHFR was significantly higher than that for either heterozygous mutation or wild homozygous mutation (P < 0.001). There was no significant difference in serum folate between groups with varied MTHFR genotypes. Plasma tHcy was slightly greater in those with lower serum folate than in those with higher one, and correlated inversely with activity of MTHFR. CONCLUSIONS: Increase in plasma tHcy correlates with homozygous mutation of thermolabile MTHFR and decrease in activity of MTHFR. Higher level of folate in the body can interfere the relationship between plasma tHcy and activity of MTHFR.
Authors: F Pangilinan; A Mitchell; J VanderMeer; A M Molloy; J Troendle; M Conley; P N Kirke; M Sutton; J M Sequeira; E V Quadros; J M Scott; J L Mills; L C Brody Journal: J Med Genet Date: 2010-06-24 Impact factor: 6.318
Authors: Anne M Molloy; Peadar N Kirke; James F Troendle; Helen Burke; Marie Sutton; Lawrence C Brody; John M Scott; James L Mills Journal: Pediatrics Date: 2009-03 Impact factor: 7.124
Authors: Faith Pangilinan; Anne M Molloy; James L Mills; James F Troendle; Anne Parle-McDermott; Caroline Signore; Valerie B O'Leary; Peter Chines; Jessica M Seay; Kerry Geiler-Samerotte; Adam Mitchell; Julia E VanderMeer; Kristine M Krebs; Angelica Sanchez; Joshua Cornman-Homonoff; Nicole Stone; Mary Conley; Peadar N Kirke; Barry Shane; John M Scott; Lawrence C Brody Journal: BMC Med Genet Date: 2012-08-02 Impact factor: 2.103