D Gao1, W Cao, X Zhang. 1. Epidemiological Department of Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Abstract
OBJECTIVE: In order to investigate the prevalence of Human ehrlichia infectious people in Daxingan Mountains. METHODS: Using PCR detection, followed by sequence analysis to detect EC and Human granulocytic ehrlichia from people in Daxingan Mountains applying the 16S rRNA gene primers of the two agents. RESULTS: Specific 16S rRNA gene sequences of EC and HGE agent were amplified from human blood specimens of tick-bitted patients and woodsmen. The detected HGE agent 16S rRNA gene (1 433 bp) amplified from a blood specimen of a tick-bitted patient differed in only one base compared with that of an American HGE agent strain. CONCLUSION: It was considered that Human ehrlichia infectious people did exist in Daxingan Mountains.
OBJECTIVE: In order to investigate the prevalence of Human ehrlichia infectious people in Daxingan Mountains. METHODS: Using PCR detection, followed by sequence analysis to detect EC and Human granulocytic ehrlichia from people in Daxingan Mountains applying the 16S rRNA gene primers of the two agents. RESULTS: Specific 16S rRNA gene sequences of EC and HGE agent were amplified from human blood specimens of tick-bitted patients and woodsmen. The detected HGE agent 16S rRNA gene (1 433 bp) amplified from a blood specimen of a tick-bitted patient differed in only one base compared with that of an American HGE agent strain. CONCLUSION: It was considered that Human ehrlichia infectious people did exist in Daxingan Mountains.
Authors: Ana Sofia Santos; Maria Margarida Santos-Silva; Victor Carlos Almeida; Fátima Bacellar; John Stephen Dumler Journal: Emerg Infect Dis Date: 2004-09 Impact factor: 6.883