Literature DB >> 1185849

Trichoplusia ni granulosis virus granulin: a phenol-soluble, phosphorylated protein.

M D Summers, G E Smith.   

Abstract

Trichoplusia ni granulosis virus granulin consists of one major polypeptide component with an estimated molecular weight of 28,000. The protein is phenol soluble, phosphorylated, and acidic. A protease activated by alkaline conditions is also associated with solubilized granulin preparations. If not properly inactivated, the protease will introduce extensive artifact into the protein giving rise to ambiguous and incorrect results as analyzed by SDS-polyacrylamide gel electrophoresis and peptide mapping. Procedures are documented for enzyme inactivation and the preparation of granulin in highly purified form for characterization.

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Year:  1975        PMID: 1185849      PMCID: PMC355708     

Source DB:  PubMed          Journal:  J Virol        ISSN: 0022-538X            Impact factor:   5.103


  18 in total

1.  Desoxyribonuclease and protease in polyhedral viral particles.

Authors:  K YAMAFUJI; J MUKAI; F YOSHIHARA
Journal:  Enzymologia       Date:  1960-06-01

2.  Phosphoproteins: structural components of oncornaviruses.

Authors:  B K Pal; P Roy-Burman
Journal:  J Virol       Date:  1975-03       Impact factor: 5.103

3.  Alkaline protease associated with the matrix protein of a virus infecting the cabbage looper.

Authors:  D A Eppstein; J A Thoma
Journal:  Biochem Biophys Res Commun       Date:  1975-01-20       Impact factor: 3.575

4.  An enzootic nuclear polyhedrosis virus of pink shrimp: ultrastructure, prevalence, and enhancement.

Authors:  J A Couch
Journal:  J Invertebr Pathol       Date:  1974-11       Impact factor: 2.841

5.  Nonhistone chromosomal proteins and gene regulation.

Authors:  G S Stein; T C Spelsberg; L J Kleinsmith
Journal:  Science       Date:  1974-03-01       Impact factor: 47.728

6.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

7.  Amino-terminal sequence analysis of proteins purified on a nanomole scale by gel electrophoresis.

Authors:  A M Weiner; T Platt; K Weber
Journal:  J Biol Chem       Date:  1972-05-25       Impact factor: 5.157

8.  Gene activation in eukaryotes: are nuclear acidic proteins the cause or the effect?

Authors:  T Pederson
Journal:  Proc Natl Acad Sci U S A       Date:  1974-03       Impact factor: 11.205

9.  Physical and chemical properties of Trichoplusia ni granulosis virus granulin.

Authors:  M D Summers; K Egawa
Journal:  J Virol       Date:  1973-11       Impact factor: 5.103

10.  Location of disulphide bridges by diagonal paper electrophoresis. The disulphide bridges of bovine chymotrypsinogen A.

Authors:  J R Brown; B S Hartley
Journal:  Biochem J       Date:  1966-10       Impact factor: 3.857

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  17 in total

1.  Determination of the protein composition of the occlusion-derived virus of Autographa californica nucleopolyhedrovirus.

Authors:  S C Braunagel; W K Russell; G Rosas-Acosta; D H Russell; M D Summers
Journal:  Proc Natl Acad Sci U S A       Date:  2003-08-06       Impact factor: 11.205

2.  Proteomics analysis of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus identified two new occlusion-derived virus-associated proteins, HA44 and HA100.

Authors:  Fei Deng; Ranran Wang; Minggang Fang; Yue Jiang; Xushi Xu; Hanzhong Wang; Xinwen Chen; Basil M Arif; Lin Guo; Hualin Wang; Zhihong Hu
Journal:  J Virol       Date:  2007-06-20       Impact factor: 5.103

3.  Comparative proteomics reveal fundamental structural and functional differences between the two progeny phenotypes of a baculovirus.

Authors:  Dianhai Hou; Leike Zhang; Fei Deng; Wei Fang; Ranran Wang; Xijia Liu; Lin Guo; Simon Rayner; Xinwen Chen; Hualin Wang; Zhihong Hu
Journal:  J Virol       Date:  2012-10-31       Impact factor: 5.103

4.  Characterization of an alkaline protease associated with a granulosis virus of Plodia interpunctella.

Authors:  K A Tweeten; L A Bulla; R A Consigli
Journal:  J Virol       Date:  1978-06       Impact factor: 5.103

5.  Characterization and serology of the matrix protein from a nuclear-polyhedrosis virus of Trichoplusia ni before and after degradation by an endogenous proteinase.

Authors:  D A Eppstein; J A Thoma
Journal:  Biochem J       Date:  1977-11-01       Impact factor: 3.857

6.  [Estimation of the molecular weight of the matrix protein of insect baculovirus inclusion bodies (author's transl)].

Authors:  G Croizier; L Croizier
Journal:  Arch Virol       Date:  1977       Impact factor: 2.574

7.  Comparison of biophysical and morphological properties of occluded and extracellular nonoccluded baculovirus from in vivo and in vitro host systems.

Authors:  M D Summers; L E Volkman
Journal:  J Virol       Date:  1976-03       Impact factor: 5.103

Review 8.  Applied and molecular aspects of insect granulosis viruses.

Authors:  K A Tweeten; L A Bulla; R A Consigli
Journal:  Microbiol Rev       Date:  1981-09

9.  Comparative properties of the inclusion body proteins of the nucleopolyhedrosis viruses of Neodiprion sertifer and Lymantria dispar.

Authors:  S E Brown; F S Kaczmarek; N R Dubois; R T Zerillo; J Holleman; J P Breillatt; H M Mazzone
Journal:  Arch Virol       Date:  1979       Impact factor: 2.574

10.  Occluded and nonoccluded nuclear polyhedrosis virus grown in Trichoplusia ni: comparative neutralization comparative infectivity, and in vitro growth studies.

Authors:  L E Volkman; M D Summers; C H Hsieh
Journal:  J Virol       Date:  1976-09       Impact factor: 5.103

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