Literature DB >> 11855569

Interaction of fibronectin and aggregation substance promotes adherence of Enterococcus faecalis to human colon.

R Isenmann1, M Schwarz, E Rozdzinski, C Christ, E Schmidt, P Augat, R Marre, H G Beger.   

Abstract

This in vitro study investigates the interaction between aggregation substance (AS), a virulence factor of Enterococcus faecalis, and colonic mucosal fibronectin in normal colon and colon from patients with Crohn's disease. Fibronectin was found to be overexpressed in Crohn's disease compared to normal colon. Compared to E. faecalis OG1X:pAM944 (AS-negative), E. faecalis OG1X:pAM721 (expressing AS) showed a significantly enhanced adhesion to human colonic mucosa in normal colon and in colon from patients with Crohn's disease. Double-staining of fibronectin and AS-positive enterococci showed that colocalization of bacteria and fibronectin was significantly more frequent in Crohn's disease than in normal colon. Preincubation of bacteria with soluble fibronectin caused a significant reduction in the adherence to fibronectin. In conclusion, the interaction between AS and fibronectin plays is an important factor that mediates adhesion of Enterococcus faecalis to colonic mucosa. This might be one of the mechanisms responsible for bacterial translocation of Enterococcus faecalis.

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Year:  2002        PMID： 11855569     DOI： 10.1023/a:1013763331708

Source DB:  PubMed          Journal:  Dig Dis Sci        ISSN： 0163-2116            Impact factor:   3.199


  34 in total

1.  Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1.

Authors:  D Galli; F Lottspeich; R Wirth
Journal:  Mol Microbiol       Date:  1990-06       Impact factor: 3.501

2.  Regulation of aggregation substance expression by bacterial and host factors.

Authors:  B A Leonard; H Hirt; G M Dunny
Journal:  Adv Exp Med Biol       Date:  1997       Impact factor: 2.622

Review 3.  Bacterial proteins binding to the mammalian extracellular matrix.

Authors:  B Westerlund; T K Korhonen
Journal:  Mol Microbiol       Date:  1993-08       Impact factor: 3.501

4.  Interactions of bacteria with leukocyte integrins.

Authors:  E Rozdzinski; E Tuomanen
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

5.  Colonic bacteria and bacterial translocation in experimental colitis.

Authors:  K R Gardiner; P J Erwin; N H Anderson; J G Barr; M I Halliday; B J Rowlands
Journal:  Br J Surg       Date:  1993-04       Impact factor: 6.939

6.  Incidence of hemolysin, gelatinase, and aggregation substance among enterococci isolated from patients with endocarditis and other infections and from feces of hospitalized and community-based persons.

Authors:  T M Coque; J E Patterson; J M Steckelberg; B E Murray
Journal:  J Infect Dis       Date:  1995-05       Impact factor: 5.226

7.  Genetic analysis of the pAD1 pheromone response in Streptococcus faecalis, using transposon Tn917 as an insertional mutagen.

Authors:  Y Ike; D B Clewell
Journal:  J Bacteriol       Date:  1984-06       Impact factor: 3.490

8.  A plasmid-encoded surface protein on Enterococcus faecalis augments its internalization by cultured intestinal epithelial cells.

Authors:  S B Olmsted; G M Dunny; S L Erlandsen; C L Wells
Journal:  J Infect Dis       Date:  1994-12       Impact factor: 5.226

9.  Altered content and distribution of tenascin in colitis, colon adenoma, and colorectal carcinoma.

Authors:  S E Riedl; A Faissner; P Schlag; A Von Herbay; K Koretz; P Möller
Journal:  Gastroenterology       Date:  1992-08       Impact factor: 22.682

10.  The prevalence of gut translocation in humans.

Authors:  P C Sedman; J Macfie; P Sagar; C J Mitchell; J May; B Mancey-Jones; D Johnstone
Journal:  Gastroenterology       Date:  1994-09       Impact factor: 22.682
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  7 in total

1.  Characterization and studies of the cellular interaction of native colonization factor CS6 purified from a clinical isolate of enterotoxigenic Escherichia coli.

Authors:  Abhisek Ghosal; Rudra Bhowmick; Rajat Banerjee; Sandipan Ganguly; S Yamasaki; T Ramamurthy; T Hamabata; Nabendu Sekhar Chatterjee
Journal:  Infect Immun       Date:  2009-02-23       Impact factor: 3.441

2.  Polarized fibronectin secretion induced by adenosine regulates bacterial-epithelial interaction in human intestinal epithelial cells.

Authors:  Baljit Walia; Florencia E Castaneda; Lixin Wang; Vasantha L Kolachala; Rahul Bajaj; Jesse Roman; Didier Merlin; Andrew T Gewirtz; Shanthi V Sitaraman
Journal:  Biochem J       Date:  2004-09-01       Impact factor: 3.857

3.  The aggregation domain of aggregation substance, not the RGD motifs, is critical for efficient internalization by HT-29 enterocytes.

Authors:  Christopher M Waters; Carol L Wells; Gary M Dunny
Journal:  Infect Immun       Date:  2003-10       Impact factor: 3.441

4.  The major pilin subunit of the AAF/II fimbriae from enteroaggregative Escherichia coli mediates binding to extracellular matrix proteins.

Authors:  Mauricio J Farfan; Keith G Inman; James P Nataro
Journal:  Infect Immun       Date:  2008-06-30       Impact factor: 3.441

5.  Microarray Analyses of Genes Differentially Expressed by Diet (Black Beans and Soy Flour) during Azoxymethane-Induced Colon Carcinogenesis in Rats.

Authors:  Elizabeth A Rondini; Maurice R Bennink
Journal:  J Nutr Metab       Date:  2012-02-08

Review 6.  Polymer Adhesin Domains in Gram-Positive Cell Surface Proteins.

Authors:  Michael A Järvå; Helmut Hirt; Gary M Dunny; Ronnie P-A Berntsson
Journal:  Front Microbiol       Date:  2020-11-26       Impact factor: 5.640

7.  The Characterization of Enterococcus Genus: Resistance Mechanisms and Inflammatory Bowel Disease.

Authors:  Michaela Růžičková; Monika Vítězová; Ivan Kushkevych
Journal:  Open Med (Wars)       Date:  2020-04-03
  7 in total

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