AIM: To study the clinicopathological and molecular genetic characteristics of typical Chinese hereditary nonpolyposis cotorectal cancer (HNPCC) families. METHODS: Four typical Chinese HNPCC families were analyzed using microdissection, microsatellite instability analysis, immunostaining of hMSH2 and hMLH1 proteins and direct DNA sequencing of hMSH2 and hMLH1 genes. RESULTS: All five tumor tissues of 4 probands from the 4 typical Chinese HNPCC families showed microsatellite instability at more than two loci (MSI-H or RER+ phenotype). Three out of the 4 cases lost hMSH2 protein expression and the other case showed no hMLH1 protein expression. Three pathological germline mutations (2 in hMSH2 and 1 in hMLH1), which had not been reported previously, were identified. The same mutations were also found in other affected members of two HNPCC families,respectively. CONCLUSION: Typical Chinese HNPCC families showed relatively frequent germline mutation of mismatch repair genes. High-level microsatellite instability and loss of expression of mismatch repair genes correlated closely with germline mutation of mismatch repair genes. Microsatellite instability analysis and immunostaining of mismatch repair gene might serve as effective screening methods before direct DNA sequencing. It is necessary to establish clinical criteria and molecular diagnostic strategies more suitable for Chinese HNPCC families.
AIM: To study the clinicopathological and molecular genetic characteristics of typical Chinese hereditary nonpolyposis cotorectal cancer (HNPCC) families. METHODS: Four typical Chinese HNPCC families were analyzed using microdissection, microsatellite instability analysis, immunostaining of hMSH2 and hMLH1 proteins and direct DNA sequencing of hMSH2 and hMLH1 genes. RESULTS: All five tumor tissues of 4 probands from the 4 typical Chinese HNPCC families showed microsatellite instability at more than two loci (MSI-H or RER+ phenotype). Three out of the 4 cases lost hMSH2 protein expression and the other case showed no hMLH1 protein expression. Three pathological germline mutations (2 in hMSH2 and 1 in hMLH1), which had not been reported previously, were identified. The same mutations were also found in other affected members of two HNPCC families,respectively. CONCLUSION: Typical Chinese HNPCC families showed relatively frequent germline mutation of mismatch repair genes. High-level microsatellite instability and loss of expression of mismatch repair genes correlated closely with germline mutation of mismatch repair genes. Microsatellite instability analysis and immunostaining of mismatch repair gene might serve as effective screening methods before direct DNA sequencing. It is necessary to establish clinical criteria and molecular diagnostic strategies more suitable for Chinese HNPCC families.
Authors: M P de Leon; M Pedroni; P Benatti; A Percesepe; C Di Gregorio; M Foroni; G Rossi; M Genuardi; G Neri; F Leonardi; A Viel; E Capozzi; M Boiocchi; L Roncucci Journal: Gut Date: 1999-07 Impact factor: 23.059
Authors: L Cawkwell; S Gray; H Murgatroyd; F Sutherland; L Haine; M Longfellow; S O'Loughlin; D Cross; O Kronborg; C Fenger; N Mapstone; M Dixon; P Quirke Journal: Gut Date: 1999-09 Impact factor: 23.059
Authors: D Calistri; S Presciuttini; G Buonsanti; P Radice; I Gazzoli; V Pensotti; P Sala; M Eboli; S Andreola; A Russo; M Pierotti; L Bertario; G N Ranzani Journal: Int J Cancer Date: 2000-01-20 Impact factor: 7.396
Authors: Y Q Bai; Y Akiyama; H Nagasaki; S L Lu; T Arai; T Morisaki; M Kitamura; A Muto; M Nagashima; T Nomizu; T Iwama; H Itoh; S Baba; T Iwai; Y Yuasa Journal: Int J Cancer Date: 1999-08-12 Impact factor: 7.396
Authors: C Lamberti; R Kruse; C Ruelfs; R Caspari; Y Wang; M Jungck; M Mathiak; H R Malayeri; W Friedl; T Sauerbruch; P Propping Journal: Gut Date: 1999-06 Impact factor: 23.059
Authors: J Gebert; M Sun; R Ridder; U Hinz; T Lehnert; P Möller; H K Schackert; C Herfarth; M von Knebel Doeberitz Journal: Int J Oncol Date: 2000-01 Impact factor: 5.650
Authors: Da-Jun Deng; Jin Zhou; Bu-Dong Zhu; Jia-Fu Ji; Jeffrey C Harper; Steven M Powell Journal: World J Gastroenterol Date: 2003-01 Impact factor: 5.742