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Literature DB >> 11854250

Influence of the alternative sigma(28) factor on virulence and flagellum expression of Legionella pneumophila.

Klaus Heuner¹, Claudia Dietrich, Carina Skriwan, Michael Steinert, Jörg Hacker.

Abstract

The fliA gene of Legionella pneumophila encoding the alternative sigma(28) factor was inactivated by introducing a kanamycin resistance cassette. Electron microscopy and Western blot analysis revealed that the fliA mutant strain is aflagellate and expresses no flagellin. Reporter gene assays indicated that the flaA promoter is not active in the fliA mutant strain. The fliA mutant strain multiplied less effectively in coculture with amoebae than the wild-type strain and was not able to replicate in coculture with Dictyostelium discoideum.

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Year: 2002 PMID： 11854250 PMCID： PMC127743 DOI： 10.1128/IAI.70.3.1604-1608.2002

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

25 in total

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26 in total

1. The LetE protein enhances expression of multiple LetA/LetS-dependent transmission traits by Legionella pneumophila.

Authors: Michael A Bachman; Michele S Swanson
Journal: Infect Immun Date: 2004-06 Impact factor: 3.441

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Authors: Michael A Bachman; Michele S Swanson
Journal: Infect Immun Date: 2004-05 Impact factor: 3.441

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Journal: J Bacteriol Date: 2004-05 Impact factor: 3.490

Review 4. ppGpp conjures bacterial virulence.

Authors: Zachary D Dalebroux; Sarah L Svensson; Erin C Gaynor; Michele S Swanson
Journal: Microbiol Mol Biol Rev Date: 2010-06 Impact factor: 11.056

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Authors: A B Molofsky; L M Shetron-Rama; Michele S Swanson
Journal: Infect Immun Date: 2005-09 Impact factor: 3.441

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Authors: Scarlett Goon; Cheryl P Ewing; Maria Lorenzo; Dawn Pattarini; Gary Majam; Patricia Guerry
Journal: Infect Immun Date: 2006-01 Impact factor: 3.441

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Authors: Jörg Mampel; Thomas Spirig; Stefan S Weber; Janus A J Haagensen; Søren Molin; Hubert Hilbi
Journal: Appl Environ Microbiol Date: 2006-04 Impact factor: 4.792