| Literature DB >> 11853762 |
Rafal Farjo1, Jindan Yu, Mohammad I Othman, Shigeo Yoshida, Sujata Sheth, Tom Glaser, Wolfgang Baehr, Anand Swaroop.
Abstract
Microarray technology can facilitate simultaneous expression analysis of thousands of genes and assist in delineating cellular pathways involved in development or disease pathogenesis. Since public databases and commercial cDNA microarrays have an under-representation of eye-expressed genes, we generated over 3000 expressed sequence tags from three unamplified mouse eye/retina cDNA libraries. These eye-expressed genes were used to produce cDNA microarrays. Methodology for printing of slides, hybridization, scanning and data analysis has been optimized. The I-gene microarrays will be useful for establishing expression profiles of the mouse eye/retina and provide a resource for defining molecular pathways involved in development, aging and disease.Entities:
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Year: 2002 PMID： 11853762 DOI： 10.1016/s0042-6989(01)00219-x
Source DB: PubMed Journal: Vision Res ISSN： 0042-6989 Impact factor: 1.886