| Literature DB >> 11853671 |
Jeffrey R Holt1, Susan K H Gillespie, D William Provance, Kavita Shah, Kevan M Shokat, David P Corey, John A Mercer, Peter G Gillespie.
Abstract
Myosin-1c (also known as myosin-Ibeta) has been proposed to mediate the slow component of adaptation by hair cells, the sensory cells of the inner ear. To test this hypothesis, we mutated tyrosine-61 of myosin-1c to glycine, conferring susceptibility to inhibition by N(6)-modified ADP analogs. We expressed the mutant myosin-1c in utricular hair cells of transgenic mice, delivered an ADP analog through a whole-cell recording pipette, and found that the analog rapidly blocked adaptation to positive and negative deflections in transgenic cells but not in wild-type cells. The speed and specificity of inhibition suggests that myosin-1c participates in adaptation in hair cells.Entities:
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Year: 2002 PMID： 11853671 DOI： 10.1016/s0092-8674(02)00629-3
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582