D Lin1, L J Qu, H Gu, Z Chen. 1. Department of Biotechnology, College of Life Sciences, Peking University, Beijing, China.
Abstract
AIMS: To clone genes of Bacillus subtilis encoding peptides that inhibit the growth of Xanthomonas orzae pv. oryzae (Xoo). METHODS AND RESULTS: A 3.1-kb DNA fragment from B. subtilis SO113 encoding peptides that inhibit the growth of Xoo (anti-Xoo, showing an inhibition zone) was isolated from a plasmid library of B. subtilis 6 GM15. Sequence analysis revealed that it contained three complete open reading frames (ORFs): ybcO, ybcS and a novel ORF designated ybcPQ. Deleting the last 96 bp of ybcS from the plasmid eliminated the anti-Xoo activity, suggesting that ybcS is required for producing the anti-Xoo activity. However, no anti-Xoo activity could be detected for the plasmid with ybcS alone. Further analysis showed that ybcO, at least, was also required to obtain the anti-Xoo activity. CONCLUSIONS: A fragment of B. subtilis has been cloned that expresses an anti-Xoo activity that requires ybcS and ybcO. SIGNIFICANCE AND IMPACT OF THE STUDY: These genes could be useful for the genetic engineering of resistance to rice bacterial diseases and for the design of new anti-Xoo biocontrol agents.
AIMS: To clone genes of Bacillus subtilis encoding peptides that inhibit the growth of Xanthomonas orzae pv. oryzae (Xoo). METHODS AND RESULTS: A 3.1-kb DNA fragment from B. subtilis SO113 encoding peptides that inhibit the growth of Xoo (anti-Xoo, showing an inhibition zone) was isolated from a plasmid library of B. subtilis 6 GM15. Sequence analysis revealed that it contained three complete open reading frames (ORFs): ybcO, ybcS and a novel ORF designated ybcPQ. Deleting the last 96 bp of ybcS from the plasmid eliminated the anti-Xoo activity, suggesting that ybcS is required for producing the anti-Xoo activity. However, no anti-Xoo activity could be detected for the plasmid with ybcS alone. Further analysis showed that ybcO, at least, was also required to obtain the anti-Xoo activity. CONCLUSIONS: A fragment of B. subtilis has been cloned that expresses an anti-Xoo activity that requires ybcS and ybcO. SIGNIFICANCE AND IMPACT OF THE STUDY: These genes could be useful for the genetic engineering of resistance to ricebacterial diseases and for the design of new anti-Xoo biocontrol agents.
Authors: Helga Westers; Peter G Braun; Lidia Westers; Haike Antelmann; Michael Hecker; Jan D H Jongbloed; Hirofumi Yoshikawa; Teruo Tanaka; Jan Maarten van Dijl; Wim J Quax Journal: Appl Environ Microbiol Date: 2005-04 Impact factor: 4.792
Authors: Wei-Ting Liu; Yu-Liang Yang; Yuquan Xu; Anne Lamsa; Nina M Haste; Jane Y Yang; Julio Ng; David Gonzalez; Craig D Ellermeier; Paul D Straight; Pavel A Pevzner; Joe Pogliano; Victor Nizet; Kit Pogliano; Pieter C Dorrestein Journal: Proc Natl Acad Sci U S A Date: 2010-08-30 Impact factor: 11.205