Literature DB >> 11848418

Epitope tagging genomic DNA using a CD-tagging Tn10 minitransposon.

C A Telmer1, P B Berget, B Ballou, R F Murphy, J W Jarvik.   

Abstract

Here, we describe an efficient system for epitope tagging cloned genes by CD tagging using a mini-Tn10 transposon delivery vector. The system was tested against a lambdaFIX genomic clone of the human nucleolin gene. Transfection of HeLa cells with the tagged gene led to the expression of both the appropriately spliced tagged transcript and the appropriately localized tagged protein.

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Year:  2002        PMID： 11848418     DOI： 10.2144/02322rr04

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  4 in total

Review 1.  From quantitative microscopy to automated image understanding.

Authors:  Kai Huang; Robert F Murphy
Journal:  J Biomed Opt       Date:  2004 Sep-Oct       Impact factor: 3.170

Review 2.  Automated interpretation of subcellular patterns in fluorescence microscope images for location proteomics.

Authors:  Xiang Chen; Meel Velliste; Robert F Murphy
Journal:  Cytometry A       Date:  2006-07       Impact factor: 4.355

3.  Fluorogen-activating proteins as biosensors of cell-surface proteins in living cells.

Authors:  John Holleran; Dara Brown; Margaret H Fuhrman; Sally A Adler; Gregory W Fisher; Jonathan W Jarvik
Journal:  Cytometry A       Date:  2010-08       Impact factor: 4.355

4.  Improved tagging strategy for protein identification in mammalian cells.

Authors:  Agnieszka Bialkowska; Xian-Yang Zhang; Jakob Reiser
Journal:  BMC Genomics       Date:  2005-09-04       Impact factor: 3.969
  4 in total

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