| Literature DB >> 11848379 |
B Johnvesly1, B R Manjunath, G R Naik.
Abstract
Thermoalkaliphilic Bacillus sp. JB-99 was grown in a 250 ml Erlenmeyer flask containing 50 ml medium containing (g/l) Pigeon pea waste 10; NaNO3, 5.0; K2HPO4, 5.0; MgSO4 x 2H2O, 0.2 and Na2CO3, 10.0. Incubations were carried out at 50 degrees C on a rotary incubator shaker for 15 h. A high level of extra cellular thermostable protease activity was observed after 24 h incubation. The optimum temperature and pH for activity were 70 degrees C and 11, respectively, so this enzyme showed stable activity at high temperature and under alkaline conditions.Entities:
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Year: 2002 PMID: 11848379 DOI: 10.1016/s0960-8524(01)00147-x
Source DB: PubMed Journal: Bioresour Technol ISSN: 0960-8524 Impact factor: 9.642