R Mesquita1, I Pires, C Saldanha, J Martins-Silva. 1. Institute of Biochemistry, Faculty of Medicine, University of Lisbon, Av. Prof. Egas Moniz, 1649-028 Lisbon, Portugal. ruimesquita@medscape.com
Abstract
PURPOSE OF THE STUDY: To determine the effects of acetylcholine and spermineNONOate--a NO donor--on RBC membrane and oxygen carrying properties. MATERIAL AND METHODS: Aliquots of venous blood from eleven healthy subjects were incubated with ACh 10(-3) M, ACh 10(-5) M, spermineNONOate 10(-5) M and spermineNONOate 10(-4) M. The following parameters were determined: erythrocyte aggregation and deformability, hematocrit, plasma pH, osmolality, K(+), Na(+), Ca(2+) concentrations, hemoglobin, oxyhemoglobin, carboxyhemoglobin and methemoglobin concentrations, oxygen saturation of hemoglobin, oxygen and carbon dioxide partial pressures and p50. RESULTS: In presence of ACh there is an increase of erythrocyte deformability, decrease of erythrocyte aggregation, plasma pH, K(+) and Na(+) concentration, increase of Ca(2+) concentration and p50. In presence of spermineNONOate there is an increase of erythrocyte deformability, plasma pH, decrease of Na(+) and Ca(2+) concentration, increase of metHb concentration and decrease of p50. CONCLUSION: Acetylcholine and spermineNONOate are able to induce changes on RBC membrane and oxygen carrying properties.
PURPOSE OF THE STUDY: To determine the effects of acetylcholine and spermineNONOate--a NO donor--on RBC membrane and oxygen carrying properties. MATERIAL AND METHODS: Aliquots of venous blood from eleven healthy subjects were incubated with ACh 10(-3) M, ACh 10(-5) M, spermineNONOate 10(-5) M and spermineNONOate 10(-4) M. The following parameters were determined: erythrocyte aggregation and deformability, hematocrit, plasma pH, osmolality, K(+), Na(+), Ca(2+) concentrations, hemoglobin, oxyhemoglobin, carboxyhemoglobin and methemoglobin concentrations, oxygen saturation of hemoglobin, oxygen and carbon dioxide partial pressures and p50. RESULTS: In presence of ACh there is an increase of erythrocyte deformability, decrease of erythrocyte aggregation, plasma pH, K(+) and Na(+) concentration, increase of Ca(2+) concentration and p50. In presence of spermineNONOate there is an increase of erythrocyte deformability, plasma pH, decrease of Na(+) and Ca(2+) concentration, increase of metHb concentration and decrease of p50. CONCLUSION:Acetylcholine and spermineNONOate are able to induce changes on RBC membrane and oxygen carrying properties.
Authors: Lukas Diederich; Tatsiana Suvorava; Roberto Sansone; T C Stevenson Keller; Frederik Barbarino; Thomas R Sutton; Christian M Kramer; Wiebke Lückstädt; Brant E Isakson; Holger Gohlke; Martin Feelisch; Malte Kelm; Miriam M Cortese-Krott Journal: Front Physiol Date: 2018-05-11 Impact factor: 4.566