PURPOSE: To investigate the gene expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3) and their high-affinity receptors (TrkA, TrkB and TrkC) in cultured human Müller cells. METHODS: The polymerase chain reaction was performed using specific primers for NGF, BDNF, NT-3, TrkA, TrkB and TrkC with complementary DNAs as the template, which were generated from poly-A+ RNA of cultured human Müller cells. RESULTS: We detected the precursor mRNA transcripts for NGF, BDNF, NT-3, TrkB and TrkC, but not TrkA. CONCLUSION: Human Müller cells can direct mRNA expressions of a number of neurotrophins which may have a neurotrophic function in the retina. An autocrine mode of action is suggested, since Müller cells not only synthesize neurotrophic factors, but also express their specific receptors. Copyright 2002 S. Karger AG, Basel
PURPOSE: To investigate the gene expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3) and their high-affinity receptors (TrkA, TrkB and TrkC) in cultured human Müller cells. METHODS: The polymerase chain reaction was performed using specific primers for NGF, BDNF, NT-3, TrkA, TrkB and TrkC with complementary DNAs as the template, which were generated from poly-A+ RNA of cultured human Müller cells. RESULTS: We detected the precursor mRNA transcripts for NGF, BDNF, NT-3, TrkB and TrkC, but not TrkA. CONCLUSION:Human Müller cells can direct mRNA expressions of a number of neurotrophins which may have a neurotrophic function in the retina. An autocrine mode of action is suggested, since Müller cells not only synthesize neurotrophic factors, but also express their specific receptors. Copyright 2002 S. Karger AG, Basel
Authors: Winston Lee; Kalev Nõupuu; Maris Oll; Tobias Duncker; Tomas Burke; Jana Zernant; Srilaxmi Bearelly; Stephen H Tsang; Janet R Sparrow; Rando Allikmets Journal: Invest Ophthalmol Vis Sci Date: 2014-08-19 Impact factor: 4.799
Authors: Danian Gu; William A Beltran; Sue Pearce-Kelling; Zexiao Li; Gregory M Acland; Gustavo D Aguirre Journal: Invest Ophthalmol Vis Sci Date: 2009-02-21 Impact factor: 4.799
Authors: Ricardo Augusto de Melo Reis; Ana Lúcia Marques Ventura; Clarissa Sampaio Schitine; Maria Christina Fialho de Mello; Fernando Garcia de Mello Journal: Neurochem Res Date: 2008-02-14 Impact factor: 3.996
Authors: Danian Gu; William A Beltran; Zexiao Li; Gregory M Acland; Gustavo D Aguirre Journal: Invest Ophthalmol Vis Sci Date: 2007-11 Impact factor: 4.799