Literature DB >> 11832508

Phage-display antibody detection of Chlamydia trachomatis-associated antigens.

Erika A Lindquist1, James D Marks, Betsy J Kleba, Richard S Stephens.   

Abstract

A phage-displayed human single-chain Fv antibody library (6.7x10(9) members) was used to select probes specific to components associated with the surface of Chlamydia trachomatis elementary bodies (EBs). Each of 15 antibodies was characterized by ELISA, dot-blot, immunoblot and immunocytochemistry, resulting in the identification of several new chlamydial components associated with the surface of EBs. In addition, six antibodies were specific for host-cell components associated with the surface of EBs. While phage display has been used effectively to produce specific antibodies for purified components, these data show that this technology is suitable for selection of specific probes from complex antigens such as the surface of a microbial pathogen.

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Year:  2002        PMID： 11832508     DOI： 10.1099/00221287-148-2-443

Source DB:  PubMed          Journal:  Microbiology        ISSN： 1350-0872            Impact factor:   2.777


  6 in total

Review 1.  Development of anti-infectives using phage display: biological agents against bacteria, viruses, and parasites.

Authors:  Johnny X Huang; Sharon L Bishop-Hurley; Matthew A Cooper
Journal:  Antimicrob Agents Chemother       Date:  2012-06-04       Impact factor: 5.191

2.  Recruitment of mammalian cell fibronectin to the surface of Chlamydia trachomatis.

Authors:  Betsy J Kleba; Erin Banta; Erika A Lindquist; Richard S Stephens
Journal:  Infect Immun       Date:  2002-07       Impact factor: 3.441

Review 3.  Developing Recombinant Antibodies by Phage Display Against Infectious Diseases and Toxins for Diagnostics and Therapy.

Authors:  Kristian Daniel Ralph Roth; Esther Veronika Wenzel; Maximilian Ruschig; Stephan Steinke; Nora Langreder; Philip Alexander Heine; Kai-Thomas Schneider; Rico Ballmann; Viola Fühner; Philipp Kuhn; Thomas Schirrmann; André Frenzel; Stefan Dübel; Maren Schubert; Gustavo Marçal Schmidt Garcia Moreira; Federico Bertoglio; Giulio Russo; Michael Hust
Journal:  Front Cell Infect Microbiol       Date:  2021-07-07       Impact factor: 5.293

Review 4.  Bacteriophages and Their Immunological Applications against Infectious Threats.

Authors:  Elena Criscuolo; Sara Spadini; Jacopo Lamanna; Mattia Ferro; Roberto Burioni
Journal:  J Immunol Res       Date:  2017-04-16       Impact factor: 4.818

5.  Bioprospecting by Phage Display of Mimetic Peptides of Chlamydia trachomatis for Use in Laboratory Diagnosis.

Authors:  Larissa Silva de Freitas; Maria Alice Freitas Queiroz; Luiz Ricardo Goulart; Luiz Fernando Almeida Machado; Antonio Carlos Rosário Vallinoto; Marluísa de Oliveira Guimarães Ishak; Fabiana de Almeida Araújo Santos; Ricardo Ishak
Journal:  Infect Drug Resist       Date:  2022-08-30       Impact factor: 4.177

Review 6.  Recombinant antibodies for diagnostics and therapy against pathogens and toxins generated by phage display.

Authors:  Philipp Kuhn; Viola Fühner; Tobias Unkauf; Gustavo Marcal Schmidt Garcia Moreira; André Frenzel; Sebastian Miethe; Michael Hust
Journal:  Proteomics Clin Appl       Date:  2016-06-21       Impact factor: 3.494
  6 in total

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