P Zhu1, W Zhang. 1. Department of Oral Pathlology, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Abstract
OBJECTIVE: To evaluate the role of matrix vesicles (MVs) during the development of mineralizing tissues. METHODS: The ultrastructure of MVs of tooth germ, calvarial bone and mandibular bone were observed by transmission electron microscopy in fetal Wistar rat. RESULTS: (1) The MVs were formed by osteoblast of the secret type, dentinoblast and process; (2) There was a mature process of the MVs in the extracellular matrix. The appearance of the hydroxyapatite (HAP) was a major mature feature; (3) HAP formed initially in the MVs entered into collage matrix and eventually mineralized; (4) The shifting sign of HAP from MVs to the surface of collage fibril was observed. CONCLUSIONS: The MVs played an important role in the biomineralization, but the special arrange pattern of mature collage fiber provided a suitable environment and a model to the growth of HAP for further mineralization.
OBJECTIVE: To evaluate the role of matrix vesicles (MVs) during the development of mineralizing tissues. METHODS: The ultrastructure of MVs of tooth germ, calvarial bone and mandibular bone were observed by transmission electron microscopy in fetal Wistar rat. RESULTS: (1) The MVs were formed by osteoblast of the secret type, dentinoblast and process; (2) There was a mature process of the MVs in the extracellular matrix. The appearance of the hydroxyapatite (HAP) was a major mature feature; (3) HAP formed initially in the MVs entered into collage matrix and eventually mineralized; (4) The shifting sign of HAP from MVs to the surface of collage fibril was observed. CONCLUSIONS: The MVs played an important role in the biomineralization, but the special arrange pattern of mature collage fiber provided a suitable environment and a model to the growth of HAP for further mineralization.