Two-hybrid arrays.

The two-hybrid system is a genetic method for detecting protein-protein interactions. The assay can be applied to random libraries or arrays of colonies that express defined pairs of proteins. Arrays enable the testing of all possible protein pairs for interactions in a systematic fashion. The array format makes a large number of individual assays comparable and thus greatly simplifies the identification of false positives. Two-hybrid arrays have been used to study interactions among the proteins of yeast, hepatitis C virus, vaccinia virus, Drosophila, Caenorhabditis elegans, mouse and other species, and have already identified thousands of interactions.