| Literature DB >> 11827456 |
Dietrich A Stephan1, Gareth R Howell, Tanya M Teslovich, Alison J Coffey, Lorie Smith, Joan E Bailey-Wilson, Lindsay Malechek, Derek Gildea, Jeffrey R Smith, Elizabeth M Gillanders, Johanna Schleutker, Ping Hu, Helen E Steingruber, Pawandeep Dhami, Christiane M Robbins, Izabela Makalowska, John D Carpten, Raman Sood, Steve Mumm, Rolland Reinbold, Tom I Bonner, Agnes Baffoe-Bonnie, Lukas Bubendorf, Mervi Heiskanen, Olli P Kallioneimi, Andreas D Baxevanis, Shirin S Joseph, Ileana Zucchi, Robert D Burk, William Isaacs, Mark T Ross, Jeffrey M Trent.
Abstract
We have recently mapped a locus for hereditary prostate cancer (termed HPCX) to the long arm of the X chromosome (Xq25-q27) through a genome-wide linkage study. Here we report the construction of an approximately 9-Mb sequence-ready bacterial clone contig map of Xq26.3-q27.3. The contig was constructed by screening BAC/PAC libraries with markers spaced at approximately 85-kb intervals. We identified overlapping clones by end-sequencing framework clones to generate 407 new sequence-tagged sites, followed by PCR verification of overlaps. Contig assembly was based on clone restriction fingerprinting and the landmark information. We identified a minimal overlap contig for genomic sequencing, which has yielded 7.7 Mb of finished sequence and 1.5 Mb of draft sequence. The transcriptional mapping effort localized 57 known and predicted genes by database searching, STS content mapping, and sequencing, followed by sequence annotation. These transcriptional units represent candidate genes for HPCX and multiple other hereditary diseases at Xq26.3-q27.3.Entities:
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Year: 2002 PMID： 11827456 DOI： 10.1006/geno.2001.6681
Source DB: PubMed Journal: Genomics ISSN： 0888-7543 Impact factor: 5.736