Literature DB >> 11826976

Improvement of the electro-transformation efficiency of facultatively alkaliphilic Bacillus pseudofirmus OF4 by high osmolarity and glycine treatment.

M Ito1, M Nagane.   

Abstract

An electro-transformation method was established for facultatively alkaliphilic Bacillus pseudofirmus OF4. A high osmolarity electroporation medium with a high electric field strength was effective. Transformation efficiency improved 110-fold in glycine-treated cells compared to non-glycine-treated cells. Under optimum conditions, the transformation efficiency was 1.69 x 10(6) transformants per microg of pUB110.

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Year:  2001        PMID： 11826976     DOI： 10.1271/bbb.65.2773

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  13 in total

1.  Physiological function of soluble cytochrome c-552 from alkaliphilic Pseudomonas alcaliphila AL15-21(T).

Authors:  Toshihede Matsuno; Kazuaki Yoshimune; Isao Yumoto
Journal:  J Bioenerg Biomembr       Date:  2011-07-16       Impact factor: 2.945

2.  Development and use of genetic system to identify genes required for efficient low-temperature growth of Psychrobacter arcticus 273-4.

Authors:  Corien Bakermans; Rudolph E Sloup; Daniel G Zarka; James M Tiedje; Michael F Thomashow
Journal:  Extremophiles       Date:  2008-09-26       Impact factor: 2.395

3.  Isolation of a minireplicon of the virulence plasmid pXO2 of Bacillus anthracis and characterization of the plasmid-encoded RepS replication protein.

Authors:  Eowyn Tinsley; Asma Naqvi; Agathe Bourgogne; Theresa M Koehler; Saleem A Khan
Journal:  J Bacteriol       Date:  2004-05       Impact factor: 3.490

4.  Protoplast transformation of recalcitrant alkaliphilic Bacillus sp. with methylated plasmid DNA and a developed hard agar regeneration medium.

Authors:  Chenghua Gao; Yanfen Xue; Yanhe Ma
Journal:  PLoS One       Date:  2011-11-23       Impact factor: 3.240

5.  Development of an efficient electroporation method for iturin A-producing Bacillus subtilis ZK.

Authors:  Zhi Zhang; Zhong-Tao Ding; Dan Shu; Di Luo; Hong Tan
Journal:  Int J Mol Sci       Date:  2015-04-01       Impact factor: 5.923

6.  Efficient fermentative production of polymer-grade D-lactate by an engineered alkaliphilic Bacillus sp. strain under non-sterile conditions.

Authors:  Nilnate Assavasirijinda; Deyong Ge; Bo Yu; Yanfen Xue; Yanhe Ma
Journal:  Microb Cell Fact       Date:  2016-01-12       Impact factor: 5.328

7.  Global Microarray Analysis of Alkaliphilic Halotolerant Bacterium Bacillus sp. N16-5 Salt Stress Adaptation.

Authors:  Liang Yin; Yanfen Xue; Yanhe Ma
Journal:  PLoS One       Date:  2015-06-01       Impact factor: 3.240

8.  Engineering of a Bacillus amyloliquefaciens Strain with High Neutral Protease Producing Capacity and Optimization of Its Fermentation Conditions.

Authors:  Hui Wang; Lian Yang; Yanhai Ping; Yingguo Bai; Huiying Luo; Huoqing Huang; Bin Yao
Journal:  PLoS One       Date:  2016-01-11       Impact factor: 3.240

9.  Sustainable biorefining in wastewater by engineered extreme alkaliphile Bacillus marmarensis.

Authors:  David G Wernick; Sammy P Pontrelli; Alexander W Pollock; James C Liao
Journal:  Sci Rep       Date:  2016-02-01       Impact factor: 4.379

10.  A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization.

Authors:  Yajian Song; Jinshan Li; Shan Meng; Liang Yin; Yanfen Xue; Yanhe Ma
Journal:  PLoS One       Date:  2016-03-15       Impact factor: 3.240
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