Literature DB >> 11824756

Transverse relaxation optimized HCN experiment for nucleic acids: combining the advantages of TROSY and MQ spin evolution.

B Brutscher1, J P Simorre.   

Abstract

A three-dimensional MQ-TROSY-HCN pulse sequence is presented which provides intra-base and sugar-to-base correlations for 13C, 15N labeled nucleic acids (RNA, DNA). The experiment simultaneously exploits the favorable relaxation properties of 1H-13C multiple quantum coherence for sugar carbons and of 13C TROSY-type spin evolution for base carbons. MQ-TROSY-HCN thus combines the advantages of MQ-HCN for sugar-to-base and TROSY-HCN for intra-base correlations in a single experiment. In addition, two slightly different implementations of the MQ-TROSY-HCN experiment ensure optimal performance for small and larger oligonucleotides, respectively. The advantages of the MQ-TROSY-HCN experiment compared to the best previous implementations of HCN are demonstrated for a 33 nucleotide RNA aptamer.

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Year:  2001        PMID： 11824756     DOI： 10.1023/a:1013398728535

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  13 in total

1.  Transverse relaxation optimized triple-resonance NMR experiments for nucleic acids.

Authors:  R Fiala; J Czernek; V Sklenár
Journal:  J Biomol NMR       Date:  2000-04       Impact factor: 2.835

Review 2.  Proton-detected heteronuclear edited and correlated nuclear magnetic resonance and nuclear Overhauser effect in solution.

Authors:  R H Griffey; A G Redfield
Journal:  Q Rev Biophys       Date:  1987-02       Impact factor: 5.318

3.  Short selective pulses for biochemical applications.

Authors:  E Kupce; J Boyd; I D Campbell
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Authors:  B Brutscher; J Boisbouvier; E Kupce; C Tisné; F Dardel; D Marion; J P Simorre
Journal:  J Biomol NMR       Date:  2001-02       Impact factor: 2.835

5.  Novel 2D and 3D multiple-quantum bi-directional HCNCH experiments for the correlation of ribose and base protons/carbons in 13C/15N labeled RNA.

Authors:  W Hu; Y Q Gosser; W Xu; D J Patel
Journal:  J Biomol NMR       Date:  2001-06       Impact factor: 2.835

6.  Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution.

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7.  An efficient procedure for assignment of the proton, carbon and nitrogen resonances in 13C/15N labeled nucleic acids.

Authors:  E P Nikonowicz; A Pardi
Journal:  J Mol Biol       Date:  1993-08-20       Impact factor: 5.469

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Authors:  S Grzesiek; A Bax
Journal:  J Biomol NMR       Date:  1995-11       Impact factor: 2.835

9.  Two- and three-dimensional HCN experiments for correlating base and sugar resonances in 15N,13C-labeled RNA oligonucleotides.

Authors:  V Sklenár; R D Peterson; M R Rejante; J Feigon
Journal:  J Biomol NMR       Date:  1993-11       Impact factor: 2.835

10.  A semiconserved residue inhibits complex formation by stabilizing interactions in the free state of a theophylline-binding RNA.

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  9 in total

1.  New applications of 2D filtered/edited NOESY for assignment and structure elucidation of RNA and RNA-protein complexes.

Authors:  Robert D Peterson; Carla A Theimer; Haihong Wu; Juli Feigon
Journal:  J Biomol NMR       Date:  2004-01       Impact factor: 2.835

2.  Sugar-to-base correlation in nucleic acids with a 5D APSY-HCNCH or two 3D APSY-HCN experiments.

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Journal:  J Biomol NMR       Date:  2011-12-04       Impact factor: 2.835

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Authors:  Hélène Van Melckebeke; Arthur Pardi; Jérôme Boisbouvier; Jean-Pierre Simorre; Bernhard Brutscher
Journal:  J Biomol NMR       Date:  2005-08       Impact factor: 2.835

4.  Combining asymmetric 13C-labeling and isotopic filter/edit NOESY: a novel strategy for rapid and logical RNA resonance assignment.

Authors:  Regan M LeBlanc; Andrew P Longhini; Stuart F J Le Grice; Bruce A Johnson; Theodore K Dayie
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6.  4D Non-uniformly sampled C,C-NOESY experiment for sequential assignment of 13C, 15N-labeled RNAs.

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Journal:  J Biomol NMR       Date:  2013-08-21       Impact factor: 2.835

7.  Longitudinal-relaxation-enhanced NMR experiments for the study of nucleic acids in solution.

Authors:  Jonathan Farjon; Jérôme Boisbouvier; Paul Schanda; Arthur Pardi; Jean-Pierre Simorre; Bernhard Brutscher
Journal:  J Am Chem Soc       Date:  2009-06-24       Impact factor: 15.419

8.  Solution structure of a purine rich hexaloop hairpin belonging to PGY/MDR1 mRNA and targeted by antisense oligonucleotides.

Authors:  Flore Joli; Nadia Bouchemal; Alain Laigle; Brigitte Hartmann; Edith Hantz
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9.  The structure of the SOLE element of oskar mRNA.

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  9 in total

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