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Literature DB >> 11823255

Quantitation of DNA sequences in environmental PCR products by a multiplexed, bead-based method.

Abstract

A first application of a multiplexed, bead-based method is described for determining the abundances of target sequences in an environmental PCR product. Target sequences as little as 0.3% of the total amount of DNA can be quantified. Tests were conducted on 16S ribosomal DNA sequences from microorganisms collected from contaminated groundwater.

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Year: 2002 PMID： 11823255 PMCID： PMC126726 DOI： 10.1128/AEM.68.2.1010-1013.2002

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

4 in total

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Authors: M A Dojka; P Hugenholtz; S K Haack; N R Pace
Journal: Appl Environ Microbiol Date: 1998-10 Impact factor: 4.792

4 in total

11 in total

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10. Locked nucleic acid pentamers as universal PCR primers for genomic DNA amplification.

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