| Literature DB >> 11823242 |
Yusuke Tamura1, Atsuko Ohkubo, Saho Iwai, Yoichiro Wada, Takeshi Shinoda, Kazuhito Arai, Shigeru Mineki, Mitsugi Iida, Hayao Taguchi.
Abstract
Two forms of NAD-dependent D-mandelate dehydrogenase (D-ManDHs) were purified from Enterococcus faecalis IAM 10071. While these two enzymes consistently exhibited high activity toward large 2-ketoacid substrates that were branched at the C3 or C4 position, they gave distinctly different K(m) and V(max) values for these substrates and had distinct molecular weights by gel electrophoresis and gel filtration.Entities:
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Year: 2002 PMID: 11823242 PMCID: PMC126676 DOI: 10.1128/AEM.68.2.947-951.2002
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792