X Ma1, D K Qiu. 1. Shanghai Institute of Digestive Disease, Shanghai Second Medical University, Affiliated Renji Hospital, 145 Shandong Zhonglu, Shanghai 200001, China. Xiongma@netease.com
Abstract
AIM: To analyze the association of HLA-DRB1 with autoimmune hepatitis (AIH) in patients from China. METHODS: In 32 patients and 48 healthy controls, polymerase chain reaction amplification with sequence-specific primers (PCR-SSP) was performed to examine the association of certain alleles or polymorphic sequences of HLA-DRB1 with AIH. RESULTS: HLA-DRB1 typing by PCR-SSP showed that DR4 had a significantly increased frequency among patients with AIH versus healthy control (46.9% versus 20.8%; relative risk = 3.35, P=0.014). In subtypes of DR4, there was a trend of increase in the gene frequency of DRB1 0405 in patients with AIH versus healthy controls (21.9% vs 6.3%, P=0.04, but P(c) =0.08). In addition, a significant increase was found in the alleles frequency encoding QRRAA from the third hyperpolymorphic region of DR4 in the patients with AIH (86.7% of DR4 positive patients vs 40.0% in DR4 positive controls, P=0.016, P(c)=0.028, RR=9.75). CONCLUSION: AIH in Chinese is associated with HLA-DR4. There is a relationship between QRRAA sequence within the third hyperpolymorphic region of the DRB allele and AIH in Chinese.
AIM: To analyze the association of HLA-DRB1 with autoimmune hepatitis (AIH) in patients from China. METHODS: In 32 patients and 48 healthy controls, polymerase chain reaction amplification with sequence-specific primers (PCR-SSP) was performed to examine the association of certain alleles or polymorphic sequences of HLA-DRB1 with AIH. RESULTS:HLA-DRB1 typing by PCR-SSP showed that DR4 had a significantly increased frequency among patients with AIH versus healthy control (46.9% versus 20.8%; relative risk = 3.35, P=0.014). In subtypes of DR4, there was a trend of increase in the gene frequency of DRB1 0405 in patients with AIH versus healthy controls (21.9% vs 6.3%, P=0.04, but P(c) =0.08). In addition, a significant increase was found in the alleles frequency encoding QRRAA from the third hyperpolymorphic region of DR4 in the patients with AIH (86.7% of DR4 positive patients vs 40.0% in DR4 positive controls, P=0.016, P(c)=0.028, RR=9.75). CONCLUSION: AIH in Chinese is associated with HLA-DR4. There is a relationship between QRRAA sequence within the third hyperpolymorphic region of the DRB allele and AIH in Chinese.
Authors: M D Strettell; P T Donaldson; L J Thomson; P J Santrach; S B Moore; A J Czaja; R Williams Journal: Gastroenterology Date: 1997-06 Impact factor: 22.682
Authors: F Alvarez; P A Berg; F B Bianchi; L Bianchi; A K Burroughs; E L Cancado; R W Chapman; W G Cooksley; A J Czaja; V J Desmet; P T Donaldson; A L Eddleston; L Fainboim; J Heathcote; J C Homberg; J H Hoofnagle; S Kakumu; E L Krawitt; I R Mackay; R N MacSween; W C Maddrey; M P Manns; I G McFarlane; K H Meyer zum Büschenfelde; M Zeniya Journal: J Hepatol Date: 1999-11 Impact factor: 25.083
Authors: A J Czaja; M D Strettell; L J Thomson; P J Santrach; S B Moore; P T Donaldson; R Williams Journal: Hepatology Date: 1997-02 Impact factor: 17.425