AIM: To identify hepatitis C virus(HCV) core protein epitopes recognized by HLA-A2 restricted cytotoxic T lymphocyte (CTL). METHODS: Utilizing the method of computer prediction followed by a 4h(51)Cr release assay confirmation. RESULTS: The results showed that peripheral blood mononuclear cells (PBMC) obtained from two HLA-A2 positive donors who were infected with HCV could lyse autologous target cells labeled with peptide "ALAHGVRAL (core 150-158)". The rates of specific lysis of the cells from the two donors were 37.5% and 15.8%, respectively. Blocking of the CTL response with anti-CD4 mAb caused no significant decrease of the specific lysis. But blocking of CTL response with anti-CD8 mAb could abolish the lysis. CONCLUSION: The peptide (core 150-158) is the candidate epitope recognized by HLAA2 restricted CTL.
AIM: To identify hepatitis C virus(HCV) core protein epitopes recognized by HLA-A2 restricted cytotoxic T lymphocyte (CTL). METHODS: Utilizing the method of computer prediction followed by a 4h(51)Cr release assay confirmation. RESULTS: The results showed that peripheral blood mononuclear cells (PBMC) obtained from two HLA-A2 positive donors who were infected with HCV could lyse autologous target cells labeled with peptide "ALAHGVRAL (core 150-158)". The rates of specific lysis of the cells from the two donors were 37.5% and 15.8%, respectively. Blocking of the CTL response with anti-CD4 mAb caused no significant decrease of the specific lysis. But blocking of CTL response with anti-CD8 mAb could abolish the lysis. CONCLUSION: The peptide (core 150-158) is the candidate epitope recognized by HLAA2 restricted CTL.
Authors: H M Diepolder; J T Gerlach; R Zachoval; R M Hoffmann; M C Jung; E A Wierenga; S Scholz; T Santantonio; M Houghton; S Southwood; A Sette; G R Pape Journal: J Virol Date: 1997-08 Impact factor: 5.103
Authors: K C Parker; M A Bednarek; L K Hull; U Utz; B Cunningham; H J Zweerink; W E Biddison; J E Coligan Journal: J Immunol Date: 1992-12-01 Impact factor: 5.422
Authors: H W Nijman; J G Houbiers; M P Vierboom; S H van der Burg; J W Drijfhout; J D'Amaro; P Kenemans; C J Melief; W M Kast Journal: Eur J Immunol Date: 1993-06 Impact factor: 5.532
Authors: J Alexander; M F Del Guercio; J D Fikes; R W Chesnut; F V Chisari; K M Chang; E Appella; A Sette Journal: Hum Immunol Date: 1998-12 Impact factor: 2.850
Authors: M U Mondelli; A Cerino; A Lisa; S Brambilla; L Segagni; A Cividini; M Bissolati; G Missale; G Bellati; A Meola; B Bruniercole; A Nicosia; G Galfrè; E Silini Journal: Hepatology Date: 1999-08 Impact factor: 17.425
Authors: K M Chang; B Rehermann; J G McHutchison; C Pasquinelli; S Southwood; A Sette; F V Chisari Journal: J Clin Invest Date: 1997-11-01 Impact factor: 14.808
Authors: S L Tsai; Y M Chen; M H Chen; C Y Huang; I S Sheen; C T Yeh; J H Huang; G C Kuo; Y F Liaw Journal: Gastroenterology Date: 1998-10 Impact factor: 22.682
Authors: A Penna; F V Chisari; A Bertoletti; G Missale; P Fowler; T Giuberti; F Fiaccadori; C Ferrari Journal: J Exp Med Date: 1991-12-01 Impact factor: 14.307