S P Guo1, W L Wang, Y Q Zhai, Y L Zhao. 1. Department of Pathology, Xijing Hospital of the Fourth Military Medical University, Xi'an, China. guoshuangping@sina.com
Abstract
AIM: In this study we investigated the relationship of the X protein of HBV and nuclear factor-kappa B (NF-kappa B) and the expression of NF-kappa B in human hepatocellular carcinoma tissues. METHODS: Immunohistochemistry SP method was used to detect the expression of NF-kappa B and the X protein of HBV in human hepatocellular carcinoma tissues of 52 cases. Gene transfection mediated by lipofectamine was used to transfect the eukaryotic expression vector pCDNA3.1-HBX of HBV x gene into human hepatocellular carcinoma cell line HCC-9204 and NF-kappa B was detected. RESULTS: NF kappa B was widely expressed in human hepatocellular carcinoma tissues in a total of 52 cases and its expression was related to the X protein of HBV. NF-kappa B was localized both in the cytoplasm and the nuclei of hepatocellular carcinoma cells in 11 cases which were positive for the X protein of HBV while in 41 cases negative for the X protein of HBV, NF-kappa B was only localized in the cytoplasm of hepatocellular carcinoma cells but translocated to the nuclei of hepatocellular carcinoma cells after the eukaryotic expression vector pCDNA3.1-HBX was transfected into HCC-9204 cells. CONCLUSION: This study strongly suggests that the nuclear factor NF-kappa B is widely expressed in hepatocellular carcinoma tissues in different styles according to the expression of the X protein of HBV. NF-kappa B is abnormally activated in hepatocellular carcinoma, which is probably related to the X protein of HBV. The X protein of HBV can activate NF-kappa B to translocate into nuclei of hepatocellular carcinoma cells.
AIM: In this study we investigated the relationship of the X protein of HBV and nuclear factor-kappa B (NF-kappa B) and the expression of NF-kappa B in humanhepatocellular carcinoma tissues. METHODS: Immunohistochemistry SP method was used to detect the expression of NF-kappa B and the X protein of HBV in humanhepatocellular carcinoma tissues of 52 cases. Gene transfection mediated by lipofectamine was used to transfect the eukaryotic expression vector pCDNA3.1-HBX of HBV x gene into humanhepatocellular carcinoma cell line HCC-9204 and NF-kappa B was detected. RESULTS:NF kappa B was widely expressed in humanhepatocellular carcinoma tissues in a total of 52 cases and its expression was related to the X protein of HBV. NF-kappa B was localized both in the cytoplasm and the nuclei of hepatocellular carcinoma cells in 11 cases which were positive for the X protein of HBV while in 41 cases negative for the X protein of HBV, NF-kappa B was only localized in the cytoplasm of hepatocellular carcinoma cells but translocated to the nuclei of hepatocellular carcinoma cells after the eukaryotic expression vector pCDNA3.1-HBX was transfected into HCC-9204 cells. CONCLUSION: This study strongly suggests that the nuclear factor NF-kappa B is widely expressed in hepatocellular carcinoma tissues in different styles according to the expression of the X protein of HBV. NF-kappa B is abnormally activated in hepatocellular carcinoma, which is probably related to the X protein of HBV. The X protein of HBV can activate NF-kappa B to translocate into nuclei of hepatocellular carcinoma cells.
Authors: D Bellavia; A F Campese; E Alesse; A Vacca; M P Felli; A Balestri; A Stoppacciaro; C Tiveron; L Tatangelo; M Giovarelli; C Gaetano; L Ruco; E S Hoffman; A C Hayday; U Lendahl; L Frati; A Gulino; I Screpanti Journal: EMBO J Date: 2000-07-03 Impact factor: 11.598