AIM:To evaluate the possibility of using cultured human hepatocytes as a bridge between bioartificial liver and liver transplantation. METHODS: In this experiment,the efficacy of extracorporeal bioartificial liver support system (EBLSS) consisting of spheriodal human liver cells and cultured hepatocytes supernatant was assessed in vivo using galactosamine induced rabbit model of fulminant hepatic failure. RESULTS: There was no difference of survival between the two groups of rabbits, but in the supported rabbits serum alanine aminotransferase, total bilirubin and creatinine were significantly lower and hepatocyte necrosis was markedly milder than those in control animals. In addition, a good viability of human liver cells was noted after the experiment. CONCLUSION: EBLSS plays a biologic role in maintaining and compensating the function of the liver.
AIM:To evaluate the possibility of using cultured human hepatocytes as a bridge between bioartificial liver and liver transplantation. METHODS: In this experiment,the efficacy of extracorporeal bioartificial liver support system (EBLSS) consisting of spheriodal human liver cells and cultured hepatocytes supernatant was assessed in vivo using galactosamine induced rabbit model of fulminant hepatic failure. RESULTS: There was no difference of survival between the two groups of rabbits, but in the supported rabbits serum alanine aminotransferase, total bilirubin and creatinine were significantly lower and hepatocyte necrosis was markedly milder than those in control animals. In addition, a good viability of human liver cells was noted after the experiment. CONCLUSION: EBLSS plays a biologic role in maintaining and compensating the function of the liver.
Authors: F D Watanabe; C R Shackleton; S M Cohen; D E Goldman; W S Arnaout; W Hewitt; S D Colquhoun; T L Fong; J M Vierling; R W Busuttil; A A Demetriou Journal: Transplant Proc Date: 1997 Feb-Mar Impact factor: 1.066
Authors: B L Blitzer; J G Waggoner; E A Jones; H R Gralnick; D Towne; J Butler; V Weise; I J Kopin; I Walters; P F Teychenne; D G Goodman; P D Berk Journal: Gastroenterology Date: 1978-04 Impact factor: 22.682
Authors: Y L Xue; S F Zhao; Y Luo; X J Li; Z P Duan; X P Chen; W G Li; X Q Huang; Y L Li; X Cui; D G Zhong; Z Y Zhang; Z Q Huang Journal: World J Gastroenterol Date: 2001-12 Impact factor: 5.742
Authors: Joshua Hefler; Braulio A Marfil-Garza; Rena L Pawlick; Darren H Freed; Constantine J Karvellas; David L Bigam; A M James Shapiro Journal: PeerJ Date: 2021-12-09 Impact factor: 2.984