AIM:To evaluale the potential role of P-selectin and anti-P-selectin monoclonal antibody (mAb) in apoptosis during hepatic/renal ischemia-reperfusion injury. METHODS: Plasma P-selectin level, hepatic/renal P-selectin expression and cell apoptosis were detected in rat model of hepatic/renal ischemia-reperfusion injury.ELISA,immunohist ochemistry and TUNEL were used. Some ischemia-reperfusion rats were treated with anti-P-selectin mAb. RESULTS: Hepatic/renal function insuffic-iency, up-regulated expression of P-selectin in plasma and hepatic/renal tissue, hepatic/renal histopathological damages and cell apoptosis were found in rats with hepatic/renal ischemia-reperfusion injury, while these changes became less conspi-cuous in animals treated with anti-P-selectin mAb. CONCLUSION: P-selectin might mediate neutrophil infiltration and cell apoptosis and contribute to hepatic/renal ischemia-reperfusion injury, anti-P-selectin mAb might be an efficient approa-ch for the prevention and treatment of hepatic/renal ischemia reperfusion injury.
AIM:To evaluale the potential role of P-selectin and anti-P-selectin monoclonal antibody (mAb) in apoptosis during hepatic/renal ischemia-reperfusion injury. METHODS: Plasma P-selectin level, hepatic/renal P-selectin expression and cell apoptosis were detected in rat model of hepatic/renal ischemia-reperfusion injury.ELISA,immunohist ochemistry and TUNEL were used. Some ischemia-reperfusion rats were treated with anti-P-selectin mAb. RESULTS:Hepatic/renal function insuffic-iency, up-regulated expression of P-selectin in plasma and hepatic/renal tissue, hepatic/renal histopathological damages and cell apoptosis were found in rats with hepatic/renal ischemia-reperfusion injury, while these changes became less conspi-cuous in animals treated with anti-P-selectin mAb. CONCLUSION:P-selectin might mediate neutrophil infiltration and cell apoptosis and contribute to hepatic/renal ischemia-reperfusion injury, anti-P-selectin mAb might be an efficient approa-ch for the prevention and treatment of hepatic/renal ischemia reperfusion injury.
Authors: M Schumer; M C Colombel; I S Sawczuk; G Gobé; J Connor; K M O'Toole; C A Olsson; G J Wise; R Buttyan Journal: Am J Pathol Date: 1992-04 Impact factor: 4.307
Authors: Y Khew-Goodall; C M Butcher; M S Litwin; S Newlands; E I Korpelainen; L M Noack; M C Berndt; A F Lopez; J R Gamble; M A Vadas Journal: Blood Date: 1996-02-15 Impact factor: 22.113