AIM:To obtain human and murine cDNAs encoding IFN-gamma inducible protein 10 (IP-10) and cytokine responsive gene-2 (Crg-2). METHODS: The encoding genes of IP-10 and Crg-2 were amplified by RT-PCR from cultured human fibroblast cells and Balb/c mouse liver treated by IFN-gamma and TNF-alpha,respectively, and cloned into plasmids of pUC19 and pGEM3Zf(+). RESULTS: The nucleotide sequences of the amplified DNA were confirmed by endonucleases digestion and sequencing. CONCLUSION: Recombinant IP-10/crg-2 gene clones with 306bp and 314bp inserts were established for further research on biological activities and ligands of hIP-10/mCrg-2.
AIM:To obtain human and murine cDNAs encoding IFN-gamma inducible protein 10 (IP-10) and cytokine responsive gene-2 (Crg-2). METHODS: The encoding genes of IP-10 and Crg-2 were amplified by RT-PCR from cultured human fibroblast cells and Balb/c mouse liver treated by IFN-gamma and TNF-alpha,respectively, and cloned into plasmids of pUC19 and pGEM3Zf(+). RESULTS: The nucleotide sequences of the amplified DNA were confirmed by endonucleases digestion and sequencing. CONCLUSION: Recombinant IP-10/crg-2 gene clones with 306bp and 314bp inserts were established for further research on biological activities and ligands of hIP-10/mCrg-2.
Authors: M P Keane; D A Arenberg; J P Lynch; R I Whyte; M D Iannettoni; M D Burdick; C A Wilke; S B Morris; M C Glass; B DiGiovine; S L Kunkel; R M Strieter Journal: J Immunol Date: 1997-08-01 Impact factor: 5.422
Authors: M Loetscher; B Gerber; P Loetscher; S A Jones; L Piali; I Clark-Lewis; M Baggiolini; B Moser Journal: J Exp Med Date: 1996-09-01 Impact factor: 14.307