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Literature DB >> 11819366

Presence of Fas and Bcl-2 proteins in BEL-7404 human hepatoma cells.

Abstract

AIM:To study the expression of Fas and Bcl-2 proteins in BEL-7404 human hepatoma cells in order to analyze the possible relationship between cell growth regulation by alpha-fetoprotein(AFP) and Fas/Bcl-2 proteins.
METHODS: BEL-7404 human hepatoma cells were maintained in RPMI 1640 medium supplemented with 10% new-born calf serum. Cells adhered to coverslips were used to detect Fas and Bcl-2 protein expression by the avidin-biotin complex (ABC) immunocytochemical assay.
RESULTS: Immunocytochemical study showed that essentially all the BEL-7404 human hepatoma cells could express Fas and Bcl-2 proteins, although in various amount. No positive staining for Fas and Bcl-2 proteins was observed when cells were incubated with non-relevant sera, to establish the specificity.
CONCLUSION: Fas apoptosis signals and Bcl-2 rescue/survival signals from apoptosis are expressed in BEL-7404 human hepatoma cells. The finding strongly implys that AFP-mediated cell apoptosis and growth enhancement are potentially associated with Fas and Bcl-2 proteins present in those cells.

Entities: Chemical Disease Gene Species

Year: 1998 PMID： 11819366 PMCID： PMC4723449 DOI： 10.3748/wjg.v4.i6.540

Source DB: PubMed Journal: World J Gastroenterol ISSN： 1007-9327 Impact factor: 5.742

13 in total

1. Induction of apoptosis in human hepatoma cells by alpha-fetoprotein.

Authors: L N Semenkova; E I Dudich; I V Dudich
Journal: Tumour Biol Date: 1997

Review 2. alpha-fetoprotein as a biologic response modifier: relevance to domain and subdomain structure.

Authors: G J Mizejewski
Journal: Proc Soc Exp Biol Med Date: 1997-09

3. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.

Authors: S M Hsu; L Raine; H Fanger
Journal: J Histochem Cytochem Date: 1981-04 Impact factor: 2.479

Presence of Fas and Bcl-2 proteins in BEL-7404 human hepatoma cells.

1. Induction of apoptosis in human hepatoma cells by alpha-fetoprotein.

Review 2. alpha-fetoprotein as a biologic response modifier: relevance to domain and subdomain structure.

3. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.

4. Cell death studies yield cancer clues.

5. Growth-regulative activity of human alpha-fetoprotein for different types of tumor and normal cells.

Review 6. [Several transcription factors participate in the functioning of the alpha-fetoprotein gene promoter].

7. Nuclear oncogenes and alpha-fetoprotein gene expression in hepatoma cell lines.

Review 8. Apoptosis. Its significance in cancer and cancer therapy.

9. [Mutation of p53 gene and expression of alphafetoprotein during hepatocarcinogenesis].

Review 10. Bcl-2 and the regulation of programmed cell death.

1. Early apoptosis in intestinal and diffuse gastric carcinomas.

2. Expression of regulating apoptosis gene and apoptosis index in primary liver cancer.

Review 3. Current gene therapy for stomach carcinoma.

4. Induction of apoptosis by TPA and VP-16 is through translocation of TR3.

5. Intravenous chemotherapy for resected gastric cancer: meta-analysis of randomized controlled trials.

6. Effect of arsenic trioxide on human hepatoma cell line BEL-7402 cultured in vitro.

7. Telomerase inhibition and telomere loss in BEL-7404 human hepatoma cells treated with doxorubicin.

8. Hypermethylation of growth arrest DNA damage-inducible gene 45 beta promoter in human hepatocellular carcinoma.

9. Study of apoptosis in human liver cancers.

10. Fas counterattack in cholangiocarcinoma: a mechanism for immune evasion in human hilar cholangiocarcinomas.