AIM:To study the relationship between intercellular adhesive molecule-1 (ICAM-1) and liver cancer metastasis and to search for factors to predict metastasis of liver cancer. METHODS: ICAM-1 expression in fresh tissues of normal liver and hepatocellular cancer (HCC) was examined by immunoperoxidase staining.The expression of ICAM-1 in human hepatoma,tumor surrounding tissues and normal livers were semiquantitatively analyzed by Dot immuno blot. Tissue ICAM-1 expression at mRNA level was detected by Northern blot. RESULTS: All 6 cases of normal liver samples were negative in anti-ICAM-1 immunohistochemical staining, 80.0% (36/45) of HCC presented various ICAM-1 expression. The number of positive cells was a little higher in large tumors,tumors with intact capsule and metastasis, but there was no significant difference. Two cases with cancer embolus also had high ICAM-1 expression.ICAM-1 concentration in HCC (13.43 ± 0.09) was higher than that in tumor surrounding tissues (5.89 ± 0.17,P <0.01) and normal livers (4.27 ± 0.21,P <0.01). It was also higher in metastasis group (20.24 ± 0.30) than in nonmetastasis group (10.23 ± 0.12,P <0.05). Northern blot analysis revealed that ICAM-1 expression at mRNA level was also higher in HCC and cancer embolus than that in tumor surrounding tissues and normal livers. CONCLUSION: Tissue ICAM-1 could indicate the growth and metastasis of HCC, and may be an index that can predict liver cancer metastasis.
AIM:To study the relationship between intercellular adhesive molecule-1 (ICAM-1) and liver cancer metastasis and to search for factors to predict metastasis of liver cancer. METHODS:ICAM-1 expression in fresh tissues of normal liver and hepatocellular cancer (HCC) was examined by immunoperoxidase staining.The expression of ICAM-1 in humanhepatoma,tumor surrounding tissues and normal livers were semiquantitatively analyzed by Dot immuno blot. Tissue ICAM-1 expression at mRNA level was detected by Northern blot. RESULTS: All 6 cases of normal liver samples were negative in anti-ICAM-1 immunohistochemical staining, 80.0% (36/45) of HCC presented various ICAM-1 expression. The number of positive cells was a little higher in large tumors,tumors with intact capsule and metastasis, but there was no significant difference. Two cases with cancer embolus also had high ICAM-1 expression.ICAM-1 concentration in HCC (13.43 ± 0.09) was higher than that in tumor surrounding tissues (5.89 ± 0.17,P <0.01) and normal livers (4.27 ± 0.21,P <0.01). It was also higher in metastasis group (20.24 ± 0.30) than in nonmetastasis group (10.23 ± 0.12,P <0.05). Northern blot analysis revealed that ICAM-1 expression at mRNA level was also higher in HCC and cancer embolus than that in tumor surrounding tissues and normal livers. CONCLUSION: Tissue ICAM-1 could indicate the growth and metastasis of HCC, and may be an index that can predict liver cancer metastasis.
Authors: M Pirisi; E Falleti; C Fabris; G Soardo; P Toniutto; D Vitulli; F Pezzetta; N Bortolotti; F Gonano; E Bartoli Journal: Am J Clin Pathol Date: 1994-11 Impact factor: 2.493
Authors: Y Shimizu; M Minemura; T Tsukishiro; Y Kashii; M Miyamoto; H Nishimori; K Higuchi; A Watanabe Journal: Hepatology Date: 1995-08 Impact factor: 17.425
Authors: Z Y Tang; F X Sun; J Tian; S L Ye; Y K Liu; K D Liu; Q Xue; J Chen; J L Xia; L X Qin; S L Sun; L Wang; J Zhou; Y Li; Z C Ma; X D Zhou; Z Q Wu; Z Y Lin; B H Yang Journal: World J Gastroenterol Date: 2001-10 Impact factor: 5.742